Bonhomme-Faivre L, Depraetere P, Savelli M P, Amdidouche D, Bizi E, Seiller M, Orbach-Arbouys S
Laboratoire de Pharmacologie, Service Pharmacie, Hôpital Paul-Brousse, Villejuif, France.
Life Sci. 2000 Jan 21;66(9):817-27. doi: 10.1016/s0024-3205(99)00654-2.
We have previously developed a charcoal suspension for injection into human breast cancers in order to facilitate their location during surgery. We observed that charcoal particles were ingested by intra and peritumoral macrophages, some of which carried the particles at some distance from the injection site. We studied the influence of the formulation parameters of the charcoal suspension for intratumoral injection on in vitro and in vivo activation and in vivo mobilization of mouse peritoneal macrophages after intra-peritoneal injection of 2 mL of each preparation. The influence of the charcoal origin (peat vs wood), granulometry, suspension vehicle (water for parenteral injection, vs saline), concentration and excipients were studied. Micronized peat charcoal in water for injection at the highest studied concentration reduced macrophage activation in vitro and in vivo. However, macrophage mobilization was weaker than after thioglycolate injection and did not seem to be charcoal dose-dependent. The additives incorporated in the charcoal suspension led in vivo to increased peritoneal macrophage activation and mobilization (mannitol, and glucose), only increased activation (polysorbate 80 and pluronic F68) or mobilization (dextran 40, egg lecithin, and cabosil), or inhibited both activation and mobilization (cremophor EL).
我们之前研发了一种用于注射到人类乳腺癌中的炭悬浮液，以便在手术过程中便于定位肿瘤。我们观察到炭颗粒被肿瘤内和肿瘤周围的巨噬细胞摄取，其中一些巨噬细胞携带颗粒远离注射部位。我们研究了瘤内注射用炭悬浮液的配方参数对腹腔注射2 mL每种制剂后小鼠腹腔巨噬细胞的体外和体内激活以及体内动员的影响。研究了炭的来源（泥炭与木材）、粒度、悬浮剂（注射用水与生理盐水）、浓度和辅料的影响。在研究的最高浓度下，注射用水中的微粉化泥炭炭在体外和体内均降低了巨噬细胞的激活。然而，巨噬细胞的动员比注射巯基乙酸盐后弱，并且似乎不依赖于炭的剂量。炭悬浮液中加入的添加剂在体内导致腹腔巨噬细胞激活和动员增加（甘露醇和葡萄糖），仅激活增加（聚山梨酯80和普朗尼克F68）或动员增加（右旋糖酐40、卵磷脂和卡波西尔），或抑制激活和动员两者（聚氧乙烯蓖麻油EL）。
