Argekar A P, Shah S J
Department of Chemistry, The Institute of Science, Mumbai, India.
J Pharm Biomed Anal. 1999 May;19(6):813-7. doi: 10.1016/s0731-7085(98)00103-4.
A new simple, precise, rapid and selective reverse phase ion pair high performance liquid chromatography (HPLC-RP) method has been developed for the simultaneous determination of cinnarizine (CINN) and domepiridone maleate (DOME) from tablets using acetonitrile-methanol-water-0.1 N sulfuric acid (37:10:48:5 v/v/v/v) containing sodium lauryl sulfate (0.01 M), as a mobile phase and a Machery Nagel nitrile column (10 microns, 25 cm x 4.0 mm i.d.) as the stationary phase. The flow of mobile phase through the column was kept at 1.0 ml min(-1) through out the analysis. Detection was carried out using a UV detector at 225 nm. The retention times for CINN and DOME were 4.73 and 9.41 min, respectively. The linearity range and percentage recoveries for CINN and DOME were 4 1000 and 60-750 microg ml(-1) and 99.90 and 99.60%, respectively.
已开发出一种新的简单、精确、快速且具选择性的反相离子对高效液相色谱法（HPLC-RP），用于同时测定片剂中的桂利嗪（CINN）和马来酸多潘立酮（DOME）。该方法以含有十二烷基硫酸钠（0.01 M）的乙腈 - 甲醇 - 水 - 0.1 N硫酸（37:10:48:5 v/v/v/v）作为流动相，以Machery Nagel腈柱（10微米，25厘米×4.0毫米内径）作为固定相。在整个分析过程中，流动相通过柱子的流速保持在1.0毫升/分钟。使用紫外检测器在225纳米波长处进行检测。CINN和DOME的保留时间分别为4.73分钟和9.41分钟。CINN和DOME的线性范围和回收率分别为4 - 1000微克/毫升和60 - 750微克/毫升，回收率分别为99.90%和99.60%。
