Walash M I, Belal F, El-Enany N, Abdelal A A
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516 Mansoura, Egypt.
J Fluoresc. 2008 Jan;18(1):61-74. doi: 10.1007/s10895-007-0238-5. Epub 2007 Sep 25.
A rapid, simple and highly sensitive second derivative synchronous fluorometric method has been developed for the simultaneous analysis of binary mixture of cinnarizine (CN) and domperidone (DOM). The method is based upon measurement of the native fluorescence of these drugs at Deltalambda=80 nm in aqueous methanol (50% V/V). The different experimental parameters affecting the native fluorescence of the studied drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the range of 0.1 to 1.3 microg mL(-1) and 0.1-3.0 microg mL(-1) for CN and DOM, respectively with lower detection limits of 0.017 and 5.77 x 10(-3) microg mL(-1) and quantification limits of 0.058 and 0.02 microg mL(-1) for CN and DOM. The proposed method was successfully applied for the determination of the studied compounds in synthetic mixtures and in commercial tablets. The results obtained were in good agreement with those obtained with reference methods. The high sensitivity attained by the synchronous fluorometric method allowed the determination of CN in real and spiked human plasma. The mean % recoveries in case of spiked human plasma (n=3) were 96.39+/-1.18 while that in real human plasma (n = 3) was 104.67+/-4.16.
已开发出一种快速、简单且高灵敏度的二阶导数同步荧光法，用于同时分析桂利嗪（CN）和多潘立酮（DOM）的二元混合物。该方法基于在水 - 甲醇（50% V/V）中，在Δλ = 80 nm处测量这些药物的天然荧光。仔细研究并优化了影响所研究药物天然荧光的不同实验参数。对于CN和DOM，荧光 - 浓度曲线在0.1至1.3 μg mL⁻¹和0.1 - 3.0 μg mL⁻¹范围内呈线性，CN的检测限较低，为0.017 μg mL⁻¹，DOM为5.77×10⁻³ μg mL⁻¹，CN和DOM的定量限分别为0.058和0.02 μg mL⁻¹。所提出的方法成功应用于合成混合物和市售片剂中所研究化合物的测定。所得结果与参考方法所得结果高度一致。同步荧光法获得的高灵敏度使得能够测定真实和加标人血浆中的CN。加标人血浆（n = 3）的平均回收率为96.39±1.18，而真实人血浆（n = 3）中的平均回收率为104.67±4.16。
