胶束电动色谱法与高效液相色谱法测定丙酸倍他米松、克霉唑及其有关物质的比较。
Comparison between micellar electrokinetic chromatography and HPLC for the determination of Betamethasone Dipropionate, Clotrimazole and their related substances.
作者信息
Lin M, Wu N
机构信息
Department of Chemistry, State University of New York at Birmingham, 13902-6000, USA.
出版信息
J Pharm Biomed Anal. 1999 May;19(6):945-54. doi: 10.1016/s0731-7085(98)00288-x.
The complete separation of a composite mixture that consisted of Betamethasone Dipropionate (BMD), Clotrimazole and their derivatives in a pharmaceutical dosage form was achieved within 15 min using sodium dodecyl sulfate (SDS) micellar electrokinetic chromatography (MEKC). For the MEKC separations, electrophoretic media consisting of SDS-phosphate buffer and various concentrations of alcohols or acetonitrile were used. The optimal condition for separating BMD, Clotrimazole and their analogues was found to be 50 mM SDS-15% acetonitrile-5% butanol at pH 7.2. The results demonstrated that the method was valid for the quantitation of BMD, Clotrimazole and analogues with selectivity and precision comparable to that of High-Performance Liquid Chromatography (HPLC).
使用十二烷基硫酸钠（SDS）胶束电动色谱法（MEKC），在15分钟内实现了药物剂型中由二丙酸倍他米松（BMD）、克霉唑及其衍生物组成的复合混合物的完全分离。对于MEKC分离，使用了由SDS - 磷酸盐缓冲液和各种浓度的醇或乙腈组成的电泳介质。发现分离BMD、克霉唑及其类似物的最佳条件是在pH 7.2时为50 mM SDS - 15%乙腈 - 5%丁醇。结果表明，该方法对于BMD、克霉唑及其类似物的定量分析是有效的，其选择性和精密度与高效液相色谱法（HPLC）相当。
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