血清中苯巴比妥的毛细管电化学酶免疫测定法（CEEI）

Capillary electrochemical enzyme immunoassay (CEEI) for phenobarbital in serum.

作者信息

Zhang J, Heineman W R, Halsall H B

机构信息

Department of Chemistry, University of Cincinnati, OH 45221-0172, USA.

出版信息

J Pharm Biomed Anal. 1999 Feb;19(1-2):145-52. doi: 10.1016/s0731-7085(98)00053-3.

DOI:10.1016/s0731-7085(98)00053-3

PMID:10698576

Abstract

A competitive heterogeneous capillary enzyme immunoassay with electrochemical detection has been developed for phenobarbital in serum. The oxidized primary antibody was attached covalently to the modified interior surface of a microcapillary (22 microl). The competition between analyte phenobarbital and alkaline phosphatase labeled phenobarbital for a limited number of antibody binding sites was complete in 1.5 h. The enzymatic product (p-aminophenol) from the catalytic conversion of the substrate (p-aminophenyl phosphate) was detected by amperometric flow injection analysis. The calibration curve for phenobarbital had a detection limit of 30 microg l(-1) (2.8 pmoles or 0.65 ng) and a range of 30-3000 microg l(-1). The assay could be used to determine the phenobarbital serum concentration in a 4 microl clinical serum sample without pretreatment.

摘要

已开发出一种用于血清中苯巴比妥检测的具有电化学检测功能的竞争性异质毛细管酶免疫分析法。氧化的一抗共价连接到微毛细管（22微升）的修饰内表面。分析物苯巴比妥与碱性磷酸酶标记的苯巴比妥对有限数量抗体结合位点的竞争在1.5小时内完成。通过安培流动注射分析检测底物（对氨基苯磷酸）催化转化产生的酶产物（对氨基酚）。苯巴比妥的校准曲线检测限为30微克/升（2.8皮摩尔或0.65纳克），范围为30 - 3000微克/升。该分析方法可用于在无需预处理的情况下测定4微升临床血清样本中的苯巴比妥血清浓度。