SecYEG组装成四聚体以形成活性蛋白质转运通道。
SecYEG assembles into a tetramer to form the active protein translocation channel.
作者信息
Manting E H, van Der Does C, Remigy H, Engel A, Driessen A J
机构信息
Department of Microbiology, Groningen Biomolecular Sciences, Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
出版信息
EMBO J. 2000 Mar 1;19(5):852-61. doi: 10.1093/emboj/19.5.852.
Abstract
Translocase mediates preprotein translocation across the Escherichia coli inner membrane. It consists of the SecYEG integral membrane protein complex and the peripheral ATPase SecA. Here we show by functional assays, negative-stain electron microscopy and mass measurements with the scanning transmission microscope that SecA recruits SecYEG complexes to form the active translocation channel. The active assembly of SecYEG has a side length of 10.5 nm and exhibits an approximately 5 nm central cavity. The mass and structure of this SecYEG as well as the subunit stoichiometry of SecA and SecY in a soluble translocase-precursor complex reveal that translocase consists of the SecA homodimer and four SecYEG complexes.
摘要
转位酶介导前体蛋白穿过大肠杆菌内膜进行转位。它由SecYEG整合膜蛋白复合物和外周ATP酶SecA组成。在这里，我们通过功能测定、负染电子显微镜以及扫描透射显微镜的质量测量表明，SecA招募SecYEG复合物以形成活性转位通道。SecYEG的活性组装体边长为10.5纳米，并呈现出一个约5纳米的中央腔。这种SecYEG的质量和结构以及可溶性转位酶 - 前体复合物中SecA和SecY的亚基化学计量比表明，转位酶由SecA同型二聚体和四个SecYEG复合物组成。
相似文献
1
SecYEG assembles into a tetramer to form the active protein translocation channel.SecYEG组装成四聚体以形成活性蛋白质转运通道。
EMBO J. 2000 Mar 1;19(5):852-61. doi: 10.1093/emboj/19.5.852.
2
The oligomeric distribution of SecYEG is altered by SecA and translocation ligands.SecA和转运配体可改变SecYEG的寡聚体分布。
J Mol Biol. 2005 Nov 25;354(2):258-71. doi: 10.1016/j.jmb.2005.09.058. Epub 2005 Oct 7.
3
Evaluating the oligomeric state of SecYEG in preprotein translocase.评估前体蛋白转运酶中SecYEG的寡聚状态。
EMBO J. 2000 Aug 15;19(16):4393-401. doi: 10.1093/emboj/19.16.4393.
4
Binding, activation and dissociation of the dimeric SecA ATPase at the dimeric SecYEG translocase.二聚体SecA ATP酶在二聚体SecYEG转运体上的结合、激活和解离。
EMBO J. 2003 Sep 1;22(17):4375-84. doi: 10.1093/emboj/cdg418.
5
Preprotein translocation by a hybrid translocase composed of Escherichia coli and Bacillus subtilis subunits.由大肠杆菌和枯草芽孢杆菌亚基组成的杂合转位酶进行的前体蛋白转位。
J Bacteriol. 1999 Nov;181(22):7021-7. doi: 10.1128/JB.181.22.7021-7027.1999.
6
Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state.SecA与SecYEG在胶束溶液中的相互作用以及膜插入状态的形成。
Biochemistry. 1998 Jan 6;37(1):201-10. doi: 10.1021/bi972105t.
7
Electron microscopic visualization of asymmetric precursor translocation intermediates: SecA functions as a dimer.电子显微镜可视化非对称前体易位中间体：SecA 作为二聚体发挥作用。
Sci China Life Sci. 2010 Sep;53(9):1049-56. doi: 10.1007/s11427-010-4061-x. Epub 2010 Nov 23.
8
SecYEG and SecA are the stoichiometric components of preprotein translocase.SecYEG和SecA是前体蛋白转运酶的化学计量组分。
J Biol Chem. 1995 Aug 25;270(34):20106-11. doi: 10.1074/jbc.270.34.20106.
9
A single amino acid substitution in SecY stabilizes the interaction with SecA.SecY 中的单个氨基酸取代可稳定与 SecA 的相互作用。
J Biol Chem. 1999 Aug 20;274(34):23868-74. doi: 10.1074/jbc.274.34.23868.
10
In vivo cross-linking of the SecA and SecY subunits of the Escherichia coli preprotein translocase.大肠杆菌前体蛋白转运酶SecA和SecY亚基的体内交联
J Bacteriol. 1997 Sep;179(18):5699-704. doi: 10.1128/jb.179.18.5699-5704.1997.
引用本文的文献
1
Dual recognition of multiple signals in bacterial outer membrane proteins enhances assembly and maintains membrane integrity.细菌外膜蛋白中多种信号的双重识别增强了组装并维持了膜的完整性。
Elife. 2024 Jan 16;12:RP90274. doi: 10.7554/eLife.90274.
2
Structural basis of BAM-mediated outer membrane β-barrel protein assembly.BAM介导的外膜β-桶状蛋白组装的结构基础
Nature. 2023 May;617(7959):185-193. doi: 10.1038/s41586-023-05988-8. Epub 2023 Apr 26.
3
Determination of the Oligomeric State of SecYEG Protein Secretion Channel Complex Using in Vivo Photo- and Disulfide Cross-linking.利用体内光交联和二硫键交联法测定SecYEG蛋白分泌通道复合物的寡聚状态
J Biol Chem. 2016 Mar 11;291(11):5997-6010. doi: 10.1074/jbc.M115.694844. Epub 2016 Jan 8.
4
The TatC component of the twin-arginine protein translocase functions as an obligate oligomer.双精氨酸蛋白转运酶的TatC组分作为一种必需寡聚体发挥作用。
Mol Microbiol. 2015 Oct;98(1):111-29. doi: 10.1111/mmi.13106. Epub 2015 Jul 22.
5
Mechanisms of Rose Bengal inhibition on SecA ATPase and ion channel activities.孟加拉玫瑰红对SecA ATP酶和离子通道活性的抑制机制。
Biochem Biophys Res Commun. 2014 Nov 14;454(2):308-12. doi: 10.1016/j.bbrc.2014.10.070. Epub 2014 Oct 19.
6
Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation.糖基化脂蛋白酶 MPIase 对于前体蛋白转运过程中 SecG 的拓扑异构反转是必需的。
Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9734-9. doi: 10.1073/pnas.1303160110. Epub 2013 May 28.
7
Dynamic structure of the translocon SecYEG in membrane: direct single molecule observations.膜中易位子 SecYEG 的动态结构：直接单分子观察。
J Biol Chem. 2013 Jun 7;288(23):16848-16854. doi: 10.1074/jbc.M113.471870. Epub 2013 Apr 22.
8
Structural characterization of the complex of SecB and metallothionein-labeled proOmpA by cryo-electron microscopy.用冷冻电镜技术对 SecB 和金属硫蛋白标记的 proOmpA 复合物进行结构分析。
PLoS One. 2012;7(10):e47015. doi: 10.1371/journal.pone.0047015. Epub 2012 Oct 4.
9
A single copy of SecYEG is sufficient for preprotein translocation.一个 SecYEG 拷贝就足以进行前体蛋白易位。
EMBO J. 2011 Sep 6;30(21):4387-97. doi: 10.1038/emboj.2011.314.
10
SecA, a remarkable nanomachine.SecA，一种非凡的纳米机器。
Cell Mol Life Sci. 2011 Jun;68(12):2053-66. doi: 10.1007/s00018-011-0681-y. Epub 2011 Apr 10.
本文引用的文献
1
Preprotein translocation by a hybrid translocase composed of Escherichia coli and Bacillus subtilis subunits.由大肠杆菌和枯草芽孢杆菌亚基组成的杂合转位酶进行的前体蛋白转位。
J Bacteriol. 1999 Nov;181(22):7021-7. doi: 10.1128/JB.181.22.7021-7027.1999.
2
Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue.枯草芽孢杆菌yvaL基因产物作为SecG同源物的功能鉴定。
J Bacteriol. 1999 Mar;181(6):1786-92. doi: 10.1128/JB.181.6.1786-1792.1999.
3
The Sec system.Sec系统。
Curr Opin Microbiol. 1998 Apr;1(2):216-22. doi: 10.1016/s1369-5274(98)80014-3.
4
Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation.SecA的膜去插入作用是质子动力依赖的蛋白质转运刺激的基础。
EMBO J. 1999 Feb 15;18(4):1049-58. doi: 10.1093/emboj/18.4.1049.
5
The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex.细菌的SecY/E易位复合体形成的通道样结构类似于真核生物的Sec61p复合体。
J Mol Biol. 1999 Jan 29;285(4):1789-800. doi: 10.1006/jmbi.1998.2413.
6
Translocase-bound SecA is largely shielded from the phospholipid acyl chains.与转位酶结合的SecA在很大程度上免受磷脂酰链的影响。
Biochemistry. 1998 Sep 1;37(35):12261-8. doi: 10.1021/bi9809021.
7
Mass Measurement in the Scanning Transmission Electron Microscope: A Powerful Tool for Studying Membrane Proteins.扫描透射电子显微镜中的质量测量：研究膜蛋白的强大工具。
J Struct Biol. 1998;121(2):219-30. doi: 10.1006/jsbi.1997.3953.
8
BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocation.结合免疫球蛋白蛋白（BiP）通过在转运之前及转运早期封闭易位子孔的腔端来维持内质网（ER）膜的通透性屏障。
Cell. 1998 Mar 20;92(6):747-58. doi: 10.1016/s0092-8674(00)81403-8.
9
Bacterial preprotein translocase: mechanism and conformational dynamics of a processive enzyme.细菌前体蛋白转位酶：一种持续性酶的机制和构象动力学
Mol Microbiol. 1998 Feb;27(3):511-8. doi: 10.1046/j.1365-2958.1998.00713.x.
10
Sec-dependent membrane protein biogenesis: SecYEG, preprotein hydrophobicity and translocation kinetics control the stop-transfer function.依赖Sec的膜蛋白生物合成：SecYEG、前体蛋白疏水性和转运动力学控制停止转运功能。
EMBO J. 1998 Feb 2;17(3):696-705. doi: 10.1093/emboj/17.3.696.
Zotero 插件