Müller SA, Engel A
Maurice E. Müller Institute for Microscopy, Biozentrum, University of Basel, Klingelbergstrasse 70, Basel, CH-4056, Switzerland
J Struct Biol. 1998;121(2):219-30. doi: 10.1006/jsbi.1997.3953.
The technique of mass measurement in the scanning transmission electron microscope is briefly presented. Results obtained for membrane proteins, with particular emphasis on the channel forming proteins, are discussed. The data illustrate the versatility of the technique which is applicable to particulate, filamentous, and sheet-like structures. When combined with composition analysis, the absolute mass values measured with the STEM allow protein stoichiometries to be unambiguously defined. Copyright 1998 Academic Press.
简要介绍了扫描透射电子显微镜中的质量测量技术。讨论了针对膜蛋白获得的结果，尤其着重于形成通道的蛋白。数据说明了该技术的多功能性，其适用于颗粒状、丝状和片状结构。当与成分分析相结合时，用扫描透射电子显微镜测量的绝对质量值能够明确确定蛋白质的化学计量。版权所有1998年学术出版社。
