枯草芽孢杆菌yvaL基因产物作为SecG同源物的功能鉴定。
Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue.
作者信息
van Wely K H, Swaving J, Broekhuizen C P, Rose M, Quax W J, Driessen A J
机构信息
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands.
出版信息
J Bacteriol. 1999 Mar;181(6):1786-92. doi: 10.1128/JB.181.6.1786-1792.1999.
Abstract
Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic analysis. Sequence comparison of the Bacillus chromosome identified a potential homologue of SecG, termed YvaL. A chromosomal disruption of the yvaL gene results in mild cold sensitivity and causes a beta-lactamase secretion defect. The cold sensitivity is exacerbated by overexpression of the secretory protein alpha-amylase, whereas growth and beta-lactamase secretion are restored by coexpression of yvaL or the E. coli secG gene. These results indicate that the yvaL gene codes for a protein that is functionally homologous to SecG.
摘要
大肠杆菌中的蛋白质输出由转位酶介导，转位酶是一种多亚基膜蛋白复合物，其外周亚基为SecA，完整膜结构域为SecY、SecE和SecG蛋白。在革兰氏阳性菌枯草芽孢杆菌中，通过遗传分析已鉴定出SecA、SecY和SecE。对枯草芽孢杆菌染色体的序列比较鉴定出一个潜在的SecG同源物，称为YvaL。yvaL基因的染色体破坏导致轻度冷敏感性，并引起β-内酰胺酶分泌缺陷。分泌蛋白α-淀粉酶的过表达会加剧冷敏感性，而yvaL或大肠杆菌secG基因的共表达可恢复生长和β-内酰胺酶分泌。这些结果表明，yvaL基因编码一种在功能上与SecG同源的蛋白质。
相似文献
1
Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue.枯草芽孢杆菌yvaL基因产物作为SecG同源物的功能鉴定。
J Bacteriol. 1999 Mar;181(6):1786-92. doi: 10.1128/JB.181.6.1786-1792.1999.
2
Preprotein translocation by a hybrid translocase composed of Escherichia coli and Bacillus subtilis subunits.由大肠杆菌和枯草芽孢杆菌亚基组成的杂合转位酶进行的前体蛋白转位。
J Bacteriol. 1999 Nov;181(22):7021-7. doi: 10.1128/JB.181.22.7021-7027.1999.
3
SecG is an auxiliary component of the protein export apparatus of Escherichia coli.SecG是大肠杆菌蛋白质输出装置的一个辅助组件。
Mol Gen Genet. 2000 Feb;263(1):131-6. doi: 10.1007/s004380050039.
4
Roles of the conserved cytoplasmic region and non-conserved carboxy-terminal region of SecE in Escherichia coli protein translocase.大肠杆菌蛋白质转运酶中SecE保守细胞质区域和非保守羧基末端区域的作用。
J Biochem. 1996 Jun;119(6):1124-30. doi: 10.1093/oxfordjournals.jbchem.a021358.
5
Preferential interaction of Sec-G with Sec-E stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane.Sec-G与Sec-E的优先相互作用稳定了大肠杆菌细胞质膜中一种不稳定的Sec-E衍生物。
Biochem Biophys Res Commun. 1995 Dec 5;217(1):217-23. doi: 10.1006/bbrc.1995.2766.
6
The secG deletion mutation of Escherichia coli is suppressed by expression of a novel regulatory gene of Bacillus subtilis.大肠杆菌的secG缺失突变可被枯草芽孢杆菌的一个新调控基因的表达所抑制。
FEBS Lett. 1996 Jul 8;389(3):281-4. doi: 10.1016/0014-5793(96)00602-3.
7
Overexpression of phosphatidylglycerophosphate synthase restores protein translocation in a secG deletion mutant of Escherichia coli at low temperature.磷脂酰甘油磷酸合酶的过表达可在低温下恢复大肠杆菌secG缺失突变体中的蛋白质转运。
FEBS Lett. 1995 May 8;364(2):157-60. doi: 10.1016/0014-5793(95)00378-m.
8
SecYEG and SecA are the stoichiometric components of preprotein translocase.SecYEG和SecA是前体蛋白转运酶的化学计量组分。
J Biol Chem. 1995 Aug 25;270(34):20106-11. doi: 10.1074/jbc.270.34.20106.
9
Influence of a Streptomyces lividans SecG functional analogue on protein secretion.天蓝色链霉菌SecG功能类似物对蛋白质分泌的影响。
Int Microbiol. 2008 Mar;11(1):25-31.
10
Identification of a gene fragment which codes for the 364 amino-terminal amino acid residues of a SecA homologue from Bacillus subtilis: further evidence for the conservation of the protein export apparatus in gram-positive and gram-negative bacteria.枯草芽孢杆菌SecA同源物364个氨基末端氨基酸残基编码基因片段的鉴定：革兰氏阳性菌和革兰氏阴性菌中蛋白质输出装置保守性的进一步证据。
Mol Gen Genet. 1991 Sep;228(3):417-23. doi: 10.1007/BF00260635.
引用本文的文献
1
Relative contributions of non-essential Sec pathway components and cell envelope-associated proteases to high-level enzyme secretion by Bacillus subtilis.枯草芽孢杆菌中非必需 Sec 途径成分和细胞包膜相关蛋白酶对高水平酶分泌的相对贡献。
Microb Cell Fact. 2020 Feb 28;19(1):52. doi: 10.1186/s12934-020-01315-2.
2
The Cellular Mechanisms that Ensure an Efficient Secretion in Streptomyces.确保链霉菌高效分泌的细胞机制。
Antibiotics (Basel). 2018 Apr 14;7(2):33. doi: 10.3390/antibiotics7020033.
3
Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus.secG 和 secY2 突变对金黄色葡萄球菌外分泌组生物发生的综合影响。
J Bacteriol. 2010 Jul;192(14):3788-800. doi: 10.1128/JB.01452-09. Epub 2010 May 14.
4
Protein transport across and into cell membranes in bacteria and archaea.细菌和古菌中蛋白质跨膜及入胞运输。
Cell Mol Life Sci. 2010 Jan;67(2):179-99. doi: 10.1007/s00018-009-0160-x. Epub 2009 Oct 10.
5
Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes.朝着将枯草芽孢杆菌开发为膜蛋白和蛋白复合物的细胞工厂迈进。
Microb Cell Fact. 2008 Apr 4;7:10. doi: 10.1186/1475-2859-7-10.
6
The SsrA-SmpB ribosome rescue system is important for growth of Bacillus subtilis at low and high temperatures.SsrA-SmpB核糖体拯救系统对枯草芽孢杆菌在低温和高温下的生长很重要。
J Bacteriol. 2007 May;189(10):3729-37. doi: 10.1128/JB.00062-07. Epub 2007 Mar 16.
7
Two additional components of the accessory sec system mediating export of the Streptococcus gordonii platelet-binding protein GspB.介导戈登氏链球菌血小板结合蛋白GspB输出的附属sec系统的另外两个组分。
J Bacteriol. 2005 Jun;187(11):3878-83. doi: 10.1128/JB.187.11.3878-3883.2005.
8
FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion.FlhF是枯草芽孢杆菌的第三种信号识别颗粒 - GTP酶，对蛋白质分泌可有可无。
J Bacteriol. 2004 Sep;186(17):5956-60. doi: 10.1128/JB.186.17.5956-5960.2004.
9
Quantitation of the capacity of the secretion apparatus and requirement for PrsA in growth and secretion of alpha-amylase in Bacillus subtilis.枯草芽孢杆菌中分泌装置的能力定量以及PrsA在α-淀粉酶生长和分泌中的需求
J Bacteriol. 2001 Mar;183(6):1881-90. doi: 10.1128/JB.183.6.1881-1890.2001.
10
Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome.枯草芽孢杆菌中信号肽依赖性蛋白质转运：基于基因组的分泌蛋白组调查
Microbiol Mol Biol Rev. 2000 Sep;64(3):515-47. doi: 10.1128/MMBR.64.3.515-547.2000.
本文引用的文献
1
Translocation of the precursor of alpha-amylase into Bacillus subtilis membrane vesicles.α-淀粉酶前体向枯草芽孢杆菌膜泡的转运
Eur J Biochem. 1998 Aug 1;255(3):690-7. doi: 10.1046/j.1432-1327.1998.2550690.x.
2
SecDF of Bacillus subtilis, a molecular Siamese twin required for the efficient secretion of proteins.枯草芽孢杆菌的SecDF，一种蛋白质高效分泌所需的分子连体双胞胎。
J Biol Chem. 1998 Aug 14;273(33):21217-24. doi: 10.1074/jbc.273.33.21217.
3
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.CLUSTAL_X 窗口界面：借助质量分析工具的多序列比对灵活策略。
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82. doi: 10.1093/nar/25.24.4876.
4
The complete genome sequence of the gram-positive bacterium Bacillus subtilis.革兰氏阳性细菌枯草芽孢杆菌的全基因组序列。
Nature. 1997 Nov 20;390(6657):249-56. doi: 10.1038/36786.
5
Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme.前体蛋白转运酶全酶的SecYE、SecG和SecDFyajC亚基的不同催化作用。
EMBO J. 1997 May 15;16(10):2756-68. doi: 10.1093/emboj/16.10.2756.
6
Isolation and characterization of a sporulation initiation mutation in the Bacillus subtilis secA gene.枯草芽孢杆菌secA基因中孢子形成起始突变的分离与鉴定
J Bacteriol. 1997 Jan;179(2):544-7. doi: 10.1128/jb.179.2.544-547.1997.
7
SecA is an intrinsic subunit of the Escherichia coli preprotein translocase and exposes its carboxyl terminus to the periplasm.SecA是大肠杆菌前体蛋白转位酶的一个内在亚基，其羧基末端暴露于周质中。
Mol Microbiol. 1996 Nov;22(4):619-29. doi: 10.1046/j.1365-2958.1996.d01-1712.x.
8
Bacillus subtilis can modulate its capacity and specificity for protein secretion through temporally controlled expression of the sipS gene for signal peptidase I.枯草芽孢杆菌可通过对信号肽酶I的sipS基因进行时间控制表达，来调节其蛋白质分泌的能力和特异性。
Mol Microbiol. 1996 Nov;22(4):605-18. doi: 10.1046/j.1365-2958.1996.d01-4676.x.
9
Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation.SecG膜拓扑结构的反转与SecA依赖性前体蛋白转运偶联。
Cell. 1996 Apr 5;85(1):71-81. doi: 10.1016/s0092-8674(00)81083-1.
10
SecA proteins of Bacillus subtilis and Escherichia coli possess homologous amino-terminal ATP-binding domains regulating integration into the plasma membrane.枯草芽孢杆菌和大肠杆菌的SecA蛋白具有同源的氨基末端ATP结合结构域，可调节其整合到质膜中。
J Bacteriol. 1995 Dec;177(24):7231-7. doi: 10.1128/jb.177.24.7231-7237.1995.
Zotero 插件