脑膜炎奈瑟菌与人上皮细胞的紧密黏附受crgA基因控制，该基因是一种新型的LysR型转录调节因子。
Intimate adhesion of Neisseria meningitidis to human epithelial cells is under the control of the crgA gene, a novel LysR-type transcriptional regulator.
作者信息
Deghmane A E, Petit S, Topilko A, Pereira Y, Giorgini D, Larribe M, Taha M K
机构信息
Unité des Neisseria, Institut Pasteur, 25, 28 Rue du Dr Roux, 75724 Paris, Cedex 15, France.
出版信息
EMBO J. 2000 Mar 1;19(5):1068-78. doi: 10.1093/emboj/19.5.1068.
Abstract
PilC1, a pilus-associated protein in Neisseria menin- gitidis, is a key element in initial meningococcal adhesion to target cells. A promoter element (CREN, contact regulatory element of Neisseria) is responsible for the transient induction of this gene upon cell contact. crgA (contact-regulated gene A) encodes a transcriptional regulator whose expression is also induced upon cell contact from a promoter region similar to the CREN of pilC1. CrgA shows significant sequence homologies to LysR-type transcriptional regulators. Its inactivation in meningococci provokes a dramatic reduction in bacterial adhesion to epithelial cells. Moreover, this mutant is unable to undergo intimate adhesion to epithelial cells or to provoke effacing of microvilli on infected cells. Purified CrgA is able to bind to pilC1 and crgA promoters, and CrgA seems to repress the expression of pilC1 and crgA. Our results support a dynamic model of bacteria-cell interaction involving a network of regulators acting in cascade. CrgA could be an intermediate regulator in such a network.
摘要
PilC1是脑膜炎奈瑟菌中一种与菌毛相关的蛋白质，是脑膜炎奈瑟菌最初黏附于靶细胞的关键因素。一种启动子元件（CREN，奈瑟菌接触调节元件）负责在细胞接触时短暂诱导该基因表达。crgA（接触调节基因A）编码一种转录调节因子，其表达在细胞接触时也从与pilC1的CREN相似的启动子区域被诱导。CrgA与LysR型转录调节因子具有显著的序列同源性。其在脑膜炎奈瑟菌中的失活导致细菌对上皮细胞的黏附显著减少。此外，该突变体无法与上皮细胞进行紧密黏附，也无法引起感染细胞微绒毛的消失。纯化的CrgA能够结合pilC1和crgA启动子，并且CrgA似乎抑制pilC1和crgA的表达。我们的结果支持一种涉及级联作用的调节因子网络的细菌 - 细胞相互作用动态模型。CrgA可能是这样一个网络中的中间调节因子。
相似文献
1
Intimate adhesion of Neisseria meningitidis to human epithelial cells is under the control of the crgA gene, a novel LysR-type transcriptional regulator.脑膜炎奈瑟菌与人上皮细胞的紧密黏附受crgA基因控制，该基因是一种新型的LysR型转录调节因子。
EMBO J. 2000 Mar 1;19(5):1068-78. doi: 10.1093/emboj/19.5.1068.
2
Analysis in vitro and in vivo of the transcriptional regulator CrgA of Neisseria meningitidis upon contact with target cells.脑膜炎奈瑟菌转录调节因子CrgA与靶细胞接触后的体外和体内分析。
Mol Microbiol. 2004 Aug;53(3):917-27. doi: 10.1111/j.1365-2958.2004.04167.x.
3
Pilus-mediated adhesion of Neisseria meningitidis is negatively controlled by the pilus-retraction machinery.菌毛介导的脑膜炎奈瑟菌黏附受菌毛回缩机制的负调控。
Mol Microbiol. 2006 Jan;59(2):579-89. doi: 10.1111/j.1365-2958.2005.04954.x.
4
Identification of a novel transcriptional regulator involved in pilC1 regulation in Neisseria meningitidis.鉴定脑膜炎奈瑟菌中参与 pilC1 调节的新型转录调节因子。
FEMS Microbiol Lett. 2010 Mar;304(2):140-7. doi: 10.1111/j.1574-6968.2009.01894.x.
5
The Neisseria meningitidis adhesion regulatory protein CrgA acts through oligomerization and interaction with RNA polymerase.脑膜炎奈瑟菌黏附调节蛋白CrgA通过寡聚化以及与RNA聚合酶相互作用发挥作用。
Mol Microbiol. 2003 Jan;47(1):135-43. doi: 10.1046/j.1365-2958.2003.03259.x.
6
Down-regulation of pili and capsule of Neisseria meningitidis upon contact with epithelial cells is mediated by CrgA regulatory protein.脑膜炎奈瑟菌与上皮细胞接触时菌毛和荚膜的下调由CrgA调节蛋白介导。
Mol Microbiol. 2002 Mar;43(6):1555-64. doi: 10.1046/j.1365-2958.2002.02838.x.
7
The phosphocarrier protein HPr of Neisseria meningitidis interacts with the transcription regulator CrgA and its deletion affects capsule production, cell adhesion, and virulence.脑膜炎奈瑟菌的磷酸载体蛋白HPr与转录调节因子CrgA相互作用，其缺失会影响荚膜产生、细胞黏附及毒力。
Mol Microbiol. 2016 Jun;100(5):788-807. doi: 10.1111/mmi.13349. Epub 2016 Mar 10.
8
The pilA regulatory gene modulates the pilus-mediated adhesion of Neisseria meningitidis by controlling the transcription of pilC1.pilA调控基因通过控制pilC1的转录来调节脑膜炎奈瑟菌菌毛介导的黏附作用。
Mol Microbiol. 1996 Mar;19(5):1073-84. doi: 10.1046/j.1365-2958.1996.448979.x.
9
CrgA is an inducible LysR-type regulator of Neisseria meningitidis, acting both as a repressor and as an activator of gene transcription.CrgA是脑膜炎奈瑟菌的一种可诱导的LysR型调节因子，它既是基因转录的阻遏物，也是激活物。
J Bacteriol. 2005 May;187(10):3421-30. doi: 10.1128/JB.187.10.3421-3430.2005.
10
The REP2 repeats of the genome of Neisseria meningitidis are associated with genes coordinately regulated during bacterial cell interaction.脑膜炎奈瑟菌基因组的REP2重复序列与细菌细胞相互作用过程中协同调控的基因相关。
J Bacteriol. 2003 Apr;185(8):2618-27. doi: 10.1128/JB.185.8.2618-2627.2003.
引用本文的文献
1
Impact of host factors and invasive meningococci on bacterial adhesion, proliferation, primary nasal epithelial barrier function, and immune response.宿主因素和侵袭性脑膜炎球菌对细菌黏附、增殖、鼻上皮初级屏障功能及免疫反应的影响。
Microbiol Spectr. 2025 Aug 5;13(8):e0014125. doi: 10.1128/spectrum.00141-25. Epub 2025 Jun 30.
2
Type IV pilin regulation: a transcriptional overview.IV型菌毛调控：转录概述。
Crit Rev Microbiol. 2025 Jun 20:1-28. doi: 10.1080/1040841X.2025.2520778.
3
Fine-tuning gene expression of regulator AdmX for improved biosynthesis of andrimid in Erwinia persicina BST187.微调调控因子AdmX的基因表达以改善桃欧文氏菌BST187中安丝菌素的生物合成。
Appl Microbiol Biotechnol. 2023 Nov;107(22):6775-6788. doi: 10.1007/s00253-023-12770-3. Epub 2023 Sep 16.
4
The Host-Pathogen Interactions and Epicellular Lifestyle of .. 的宿主-病原体相互作用和上皮细胞生活方式
Front Cell Infect Microbiol. 2022 Apr 22;12:862935. doi: 10.3389/fcimb.2022.862935. eCollection 2022.
5
Identification of a Novel LysR-Type Transcriptional Regulator in Staphylococcus aureus That Is Crucial for Secondary Tissue Colonization during Metastatic Bloodstream Infection.金黄色葡萄球菌中新型 LysR 型转录调控因子的鉴定，该因子对转移性血流感染中的次级组织定植至关重要。
mBio. 2020 Aug 25;11(4):e01646-20. doi: 10.1128/mBio.01646-20.
6
CrgA Protein Represses AlkB2 Monooxygenase and Regulates the Degradation of Medium-to-Long-Chain -Alkanes in SJTD-1.CrgA蛋白抑制AlkB2单加氧酶并调节SJTD-1中长链烷烃的降解。
Front Microbiol. 2019 Mar 12;10:400. doi: 10.3389/fmicb.2019.00400. eCollection 2019.
7
Host cell-derived lactate functions as an effector molecule in Neisseria meningitidis microcolony dispersal.宿主细胞衍生的乳酸作为脑膜炎奈瑟菌微菌落扩散中的效应分子发挥作用。
PLoS Pathog. 2017 Apr 6;13(4):e1006251. doi: 10.1371/journal.ppat.1006251. eCollection 2017 Apr.
8
The FinR-regulated essential gene fprA, encoding ferredoxin NADP+ reductase: Roles in superoxide-mediated stress protection and virulence of Pseudomonas aeruginosa.受FinR调控的必需基因fprA，编码铁氧化还原蛋白NADP+还原酶：在铜绿假单胞菌超氧化物介导的应激保护和毒力中的作用。
PLoS One. 2017 Feb 10;12(2):e0172071. doi: 10.1371/journal.pone.0172071. eCollection 2017.
9
Modulation of Kingella kingae adherence to human epithelial cells by type IV Pili, capsule, and a novel trimeric autotransporter.通过 IV 型菌毛、荚膜和一种新型三聚体自转运蛋白调节金氏金氏菌对人上皮细胞的黏附
mBio. 2012 Oct 23;3(5):e00372-12. doi: 10.1128/mBio.00372-12.
10
Late repression of NF-κB activity by invasive but not non-invasive meningococcal isolates is required to display apoptosis of epithelial cells.侵袭性而非非侵袭性脑膜炎奈瑟菌分离株晚期抑制 NF-κB 活性是显示上皮细胞凋亡所必需的。
PLoS Pathog. 2011 Dec;7(12):e1002403. doi: 10.1371/journal.ppat.1002403. Epub 2011 Dec 1.
本文引用的文献
1
NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.淋病奈瑟菌。一、与克隆变异基因连锁的毒力。
J Bacteriol. 1963 Jun;85(6):1274-9. doi: 10.1128/jb.85.6.1274-1279.1963.
2
Neisseria meningitidis induces the expression of the TNF-alpha gene in endothelial cells.脑膜炎奈瑟菌可诱导内皮细胞中肿瘤坏死因子-α基因的表达。
Cytokine. 2000 Jan;12(1):21-5. doi: 10.1006/cyto.1999.0506.
3
Type IV pili of pathogenic Neisseriae elicit cortical plaque formation in epithelial cells.致病性奈瑟菌的IV型菌毛可诱导上皮细胞形成皮质斑块。
Mol Microbiol. 1999 Jun;32(6):1316-32. doi: 10.1046/j.1365-2958.1999.01459.x.
4
The meningococcal PilT protein is required for induction of intimate attachment to epithelial cells following pilus-mediated adhesion.脑膜炎球菌的PilT蛋白是菌毛介导黏附后诱导与上皮细胞紧密附着所必需的。
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):4017-22. doi: 10.1073/pnas.96.7.4017.
5
Fimbriae-mediated host-pathogen cross-talk.菌毛介导的宿主-病原体相互作用。
Curr Opin Microbiol. 1998 Feb;1(1):75-81. doi: 10.1016/s1369-5274(98)80145-8.
6
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.CtsR是应激和热休克反应的一种新型调节因子，可控制革兰氏阳性菌中clp和分子伴侣基因的表达。
Mol Microbiol. 1999 Jan;31(1):117-31. doi: 10.1046/j.1365-2958.1999.01152.x.
7
Cell signaling by the type IV pili of pathogenic Neisseria.致病性奈瑟菌IV型菌毛介导的细胞信号传导
J Biol Chem. 1998 Aug 21;273(34):21777-82. doi: 10.1074/jbc.273.34.21777.
8
Pilus-mediated adhesion of Neisseria meningitidis: the essential role of cell contact-dependent transcriptional upregulation of the PilC1 protein.菌毛介导的脑膜炎奈瑟菌黏附作用：PilC1蛋白细胞接触依赖性转录上调的关键作用
Mol Microbiol. 1998 Jun;28(6):1153-63. doi: 10.1046/j.1365-2958.1998.00876.x.
9
10
Interaction of Neisseria meningitidis with a polarized monolayer of epithelial cells.脑膜炎奈瑟菌与极化上皮细胞单层的相互作用。
Infect Immun. 1997 Nov;65(11):4836-42. doi: 10.1128/iai.65.11.4836-4842.1997.
Zotero 插件