Schuster S, Fell D A, Dandekar T
Department of Bioinformatics, Max Delbrück Center for Molecular Medicine, D-13092 Berlin-Buch, Germany.
Nat Biotechnol. 2000 Mar;18(3):326-32. doi: 10.1038/73786.
A set of linear pathways often does not capture the full range of behaviors of a metabolic network. The concept of 'elementary flux modes' provides a mathematical tool to define and comprehensively describe all metabolic routes that are both stoichiometrically and thermodynamically feasible for a group of enzymes. We have used this concept to analyze the interplay between the pentose phosphate pathway (PPP) and glycolysis. The set of elementary modes for this system involves conventional glycolysis, a futile cycle, all the modes of PPP function described in biochemistry textbooks, and additional modes that are a priori equally entitled to pathway status. Applications include maximizing product yield in amino acid and antibiotic synthesis, reconstruction and consistency checks of metabolism from genome data, analysis of enzyme deficiencies, and drug target identification in metabolic networks.
一组线性途径往往无法涵盖代谢网络的全部行为。“基本通量模式”的概念提供了一种数学工具，用于定义和全面描述一组酶在化学计量和热力学上可行的所有代谢途径。我们已使用这一概念来分析磷酸戊糖途径（PPP）与糖酵解之间的相互作用。该系统的基本模式集包括传统糖酵解、一个无效循环、生物化学教科书中描述的PPP功能的所有模式，以及在途径状态方面同样具有先验资格的其他模式。其应用包括在氨基酸和抗生素合成中最大化产物产量、根据基因组数据重建和检查代谢的一致性、分析酶缺陷以及识别代谢网络中的药物靶点。
