Moussa M, Le Boucher J, Garcia J, Tkaczuk J, Ragab J, Dutot G, Ohayon E, Ghisolfi J, Thouvenot J P
Groupe d'Etudes en Nutrition Infantile, Toulouse, France.
Clin Nutr. 2000 Feb;19(1):49-54. doi: 10.1054/clnu.1999.0076.
Numerous studies suggest that immune function may be compromised by lipid emulsions rich in polyunsaturated fatty acids, especially linoleic acid. In our study, we compared the effect of a new olive oil-based lipid emulsion (ClinOleic(R)) containing 18% linoleic acid, and an emulsion based on soybean oil (Ivelip(R); 52% linoleic acid) on lymphocyte functions. Weaning Wistar rats (n= 24) were fed for 4 weeks on an oral diet that contained 12% of total energy as lipids from soybean oil. Then they received, during 6 days, a total parenteral nutrition (260 kcal/kg/d) in which 12% of total energy was brought by one of the two lipid emulsions. The fatty acid profile of spleen lymphocyte phospholipids reflected lipid intakes, with a higher content of oleic acid in ClinOleic(R) group and linoleic acid in Ivelip(R) group. A greater proportion of cells expressed the interleukin-2 receptor a-chain (CD25) after administration of ClinOleic(R) when compared to Ivelip(R) (55.43 +/- 3.47 vs 45.48 +/- 3.26%, P<< 0.05). Moreover, the CD25 expression was positively correlated with oleic acid content of spleen lymphocyte phospholipids (r= 0.500, P<< 0.018). These results show that ClinOleic(R) is able to induce, in vivo, a greater proportion of cells expressing CD25, and suggest that oleic acid could have a role in the observed effects.
大量研究表明，富含多不饱和脂肪酸尤其是亚油酸的脂质乳剂可能会损害免疫功能。在我们的研究中，我们比较了一种含18%亚油酸的新型橄榄油基脂质乳剂（ClinOleic®）和一种基于大豆油的乳剂（Ivelip®；含52%亚油酸）对淋巴细胞功能的影响。将断乳的Wistar大鼠（n = 24）用一种口服饮食喂养4周，该饮食中脂质提供的能量占总能量的12%，脂质来自大豆油。然后在6天内给予它们全胃肠外营养（260千卡/千克/天），其中两种脂质乳剂之一提供总能量的12%。脾淋巴细胞磷脂的脂肪酸谱反映了脂质摄入情况，ClinOleic®组油酸含量较高，Ivelip®组亚油酸含量较高。与Ivelip®相比，给予ClinOleic®后表达白细胞介素-2受体α链（CD25）的细胞比例更高（55.43±3.47对45.48±3.26%，P << 0.05）。此外，CD25表达与脾淋巴细胞磷脂的油酸含量呈正相关（r = 0.500，P << 0.018）。这些结果表明，ClinOleic®能够在体内诱导更大比例的细胞表达CD25，并提示油酸可能在观察到的效应中起作用。
