López F J, Alvariño J M
Departamento Producción Animal, E.T.S.I. Agrónomos, 28040, Madrid, Spain.
Anim Reprod Sci. 2000 Feb 28;58(1-2):147-54. doi: 10.1016/s0378-4320(99)00084-6.
Lactating rabbits (n=1335) were artificially inseminated to study the effect of the addition of caffeine on rabbit semen stored for up to 96 h. Concentration of 0-5 mM/l were tested. Whereas a concentration of 0.2 mM/l increased spermatozoa motility, higher concentration values adversely affected reproductive parameters. Spermatozoa stored at 18 degrees C for 72-96 h did not have the capacity to react with caffeine when it was added before insemination. Caffeine did not enhance fertility or prolificacy, regardless of its ability to increase sperm motility.
为研究添加咖啡因对储存长达96小时的兔精液的影响，对1335只泌乳期兔子进行了人工授精。测试了0至5 mM/l的浓度。0.2 mM/l的浓度可提高精子活力，而更高的浓度值则对生殖参数产生不利影响。在18摄氏度下储存72至96小时的精子在授精前添加咖啡因时没有与咖啡因发生反应的能力。无论咖啡因增加精子活力的能力如何，它都不会提高生育力或产仔数。
