Casares-Crespo L, Fernández-Serrano P, Vicente J S, Mocé E, Castellini C, Stabile A M, Viudes-de-Castro M P
Animal Technology and Research Center (CITA), Instituto Valenciano de Investigaciones Agrarias (IVIA), Polígono La Esperanza nº 100, 12400 Segorbe, Castellón, Spain.
Institute of Science and Animal Technology (ICTA), Universitat Politècnica de València, 46022 Valencia, Spain.
Theriogenology. 2018 Jan 1;105:61-65. doi: 10.1016/j.theriogenology.2017.09.009. Epub 2017 Sep 9.
The addition of aminopeptidase inhibitors (AMIs) to rabbit semen extenders could be a solution to decrease the hormone degradation (GnRH) by the aminopeptidases existing in the seminal plasma. Therefore, the quantity of GnRH needed to induce ovulation in doe would be comparable with the amount administered intramuscularly (i.m.). This study was conducted to evaluate the effects of two AMIs (bestatin and EDTA) on rabbit semen quality parameters, β nerve growth factor (β-NGF) degradation and reproductive performance after artificial insemination. Results showed that seminal quality was not affected by the incubation with AMIs; the values of motility, acrosome integrity and sperm viability were not significantly different between the AMIs and the control groups (positive i.m. and negative intravaginally without AMIs). In addition, the aminopeptidase activity of seminal plasma was inhibited in a 55.5% by the AMIs as well as β-NGF degradation. On the other hand, regarding the effect of AMIs on reproductive performance, our results showed that the presence of bestatin and EDTA did neither affect fertility (85.3 vs. 88.6%), nor the prolificacy rate (10.12 vs. 10.51 kits per delivery), comparing AMIs group to positive control group, respectively. We conclude that the addition of specific AMIs in the rabbit semen extender has no effect on reproductive performance. Therefore, due to the fact that AMIs inhibit part of the aminopeptidase activity that degrades the GnRH analogue and β-NGF, they could be used to develop new extenders with less hormone concentration.
在兔精液稀释液中添加氨肽酶抑制剂（AMIs）可能是减少精浆中存在的氨肽酶对激素（GnRH）降解的一种解决方案。因此，诱导母兔排卵所需的GnRH量将与肌肉注射（i.m.）的量相当。本研究旨在评估两种AMIs（抑氨肽酶素和乙二胺四乙酸）对兔精液质量参数、β神经生长因子（β-NGF）降解以及人工授精后繁殖性能的影响。结果表明，与AMIs孵育不会影响精液质量；AMIs组与对照组（肌肉注射阳性组和阴道内无AMIs阴性组）之间的活力、顶体完整性和精子活力值无显著差异。此外，AMIs对精浆氨肽酶活性以及β-NGF降解的抑制率为55.5%。另一方面，关于AMIs对繁殖性能的影响，我们的结果表明，将AMIs组与阳性对照组相比，抑氨肽酶素和乙二胺四乙酸的存在既不影响生育率（分别为85.3%对88.6%），也不影响产仔率（每次分娩分别为10.12只对10.51只）。我们得出结论，在兔精液稀释液中添加特定的AMIs对繁殖性能没有影响。因此，由于AMIs抑制了部分降解GnRH类似物和β-NGF的氨肽酶活性，它们可用于开发激素浓度更低的新型稀释液。
