Clustering of clinical strains of Helicobacter pylori analyzed by two-dimensional gel electrophoresis.
作者信息
Enroth H, Akerlund T, Sillén A, Engstrand L
机构信息
Department of Medical Epidemiology, Karolinska Institute, Stockholm, Sweden.
出版信息
Clin Diagn Lab Immunol. 2000 Mar;7(2):301-6. doi: 10.1128/CDLI.7.2.301-306.2000.
Abstract
Strain variations of Helicobacter pylori have been tested by numerous methods and compared among different patient groups. The aim of this study was to investigate whether H. pylori expresses disease-specific proteins that can be detected by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). H. pylori strains isolated from duodenal ulcer, gastric cancer, and gastritis patients were analyzed. Extensive variation in spot patterns was observed between the strains, but a dendrogram analysis revealed that some strains within each disease group clustered together. Eight proteins were sequenced and found in the H. pylori genome sequence. 2-D PAGE is a useful method for studies of protein expression and for highlighting the extensive strain variation that H. pylori exhibits.
摘要
相似文献
1
Clustering of clinical strains of Helicobacter pylori analyzed by two-dimensional gel electrophoresis.
Clin Diagn Lab Immunol. 2000 Mar;7(2):301-6. doi: 10.1128/CDLI.7.2.301-306.2000.
2
Comparative analysis of two-dimensional electrophoresis maps (2-DE) of Helicobacter pylori from Brazilian patients with chronic gastritis and duodenal ulcer: a preliminary report.
Rev Inst Med Trop Sao Paulo. 2006 May-Jun;48(3):175-7. doi: 10.1590/s0036-46652006000300011. Epub 2006 Jul 5.
3
Quantitative analysis of representative proteome components and clustering of Helicobacter pylori clinical strains.
Helicobacter. 2006 Dec;11(6):533-43. doi: 10.1111/j.1523-5378.2006.00456.x.
4
Comparative proteomics and immunoproteomics of Helicobacter pylori related to different gastric pathologies.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Mar 20;833(1):63-79. doi: 10.1016/j.jchromb.2005.12.052. Epub 2006 Feb 17.
5
Molecular relationships of Helicobacter pylori strains in a family with gastroduodenal disease.
Am J Gastroenterol. 1999 Feb;94(2):364-8. doi: 10.1111/j.1572-0241.1999.859_u.x.
6
Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis.
J Zhejiang Univ Sci B. 2011 Oct;12(10):820-7. doi: 10.1631/jzus.B1000445.
7
Isolation and characterization of Helicobacter pylori strains from peptic ulcer patients in Dhaka, Bangladesh.
Indian J Gastroenterol. 1995 Oct;14(4):128-30.
8
Presence of the cagA gene in the majority of Helicobacter pylori strains is independent of whether the individual has duodenal ulcer or asymptomatic gastritis.
Helicobacter. 1996 Jun;1(2):107-11. doi: 10.1111/j.1523-5378.1996.tb00019.x.
9
Analysis of Helicobacter pylori vacA and cagA genotypes and serum antibody profile in benign and malignant gastroduodenal diseases.
Gut. 1998 Aug;43(2):182-6. doi: 10.1136/gut.43.2.182.
10
Relationship between the diversity of the cagA gene of Helicobacter pylori and gastric cancer in Okinawa, Japan.
J Gastroenterol. 2006 Jul;41(7):668-73. doi: 10.1007/s00535-006-1838-6.
引用本文的文献
1
Differential proteomics of Helicobacter pylori associated with autoimmune atrophic gastritis.
Mol Med. 2014 Feb 28;20(1):57-71. doi: 10.2119/molmed.2013.00076.
2
Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis.
J Zhejiang Univ Sci B. 2011 Oct;12(10):820-7. doi: 10.1631/jzus.B1000445.
3
Comparative proteomic analysis of Lactobacillus plantarum for the identification of key proteins in bile tolerance.
BMC Microbiol. 2011 Mar 29;11:63. doi: 10.1186/1471-2180-11-63.
4
Proteomic comparison of the cytosolic proteins of three Bifidobacterium longum human isolates and B. longum NCC2705.
BMC Microbiol. 2010 Jan 29;10:29. doi: 10.1186/1471-2180-10-29.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
Isolation of a Helicobacter pylori protein, FldA, associated with mucosa-associated lymphoid tissue lymphoma of the stomach.
Gastroenterology. 1999 Jul;117(1):82-8. doi: 10.1016/s0016-5085(99)70553-6.
3
In a world of black and white, Helicobacter pylori is gray.
Ann Intern Med. 1999 Apr 20;130(8):695-7. doi: 10.7326/0003-4819-130-8-199904200-00019.
4
One stomach--one strain: does Helicobacter pylori strain variation influence disease outcome?
Dig Dis Sci. 1999 Jan;44(1):102-7. doi: 10.1023/a:1026658301825.
5
Identification of potential diagnostic and vaccine candidates of Helicobacter pylori by "proteome" technologies.
Helicobacter. 1998 Sep;3(3):163-9.
6
REP-PCR fragments as biomarkers for differentiating gastroduodenal disease-specific Helicobacter pylori strains.
Dig Dis Sci. 1998 May;43(5):980-7. doi: 10.1023/a:1018818431828.
7
Panmictic structure of Helicobacter pylori demonstrated by the comparative study of six genetic markers.
FEMS Microbiol Lett. 1998 Apr 15;161(2):231-9. doi: 10.1111/j.1574-6968.1998.tb12953.x.
8
Antibody to a Helicobacter pylori species specific antigen in patients with adenocarcinoma of the stomach.
Biochem Biophys Res Commun. 1998 Mar 17;244(2):360-3. doi: 10.1006/bbrc.1998.8271.
9
Genetic relationship among isolates of Helicobacter pylori: evidence for the existence of a Helicobacter pylori species-complex.
FEMS Microbiol Lett. 1997 May 1;150(1):27-32. doi: 10.1111/j.1574-6968.1997.tb10345.x.
10
Not all Helicobacter pylori strains are created equal: should all be eliminated?
Lancet. 1997 Apr 5;349(9057):1020-2. doi: 10.1016/S0140-6736(96)09133-7.
Zotero 插件