Enroth H, Nyrén O, Engstrand L
Department of Medical Epidemiology, Karolinska Institute, Stockholm, Sweden.
Dig Dis Sci. 1999 Jan;44(1):102-7. doi: 10.1023/a:1026658301825.
The aim of the study was to determine inter- and intrapatient variation of Helicobacter pylori strains based on genomic fingerprinting and cagA (cytotoxin-associated gene A) status. Ten bacterial colonies from each of 10 patients with gastric cancer (GC), 10 with duodenal ulcer (DU), and 10 with gastritis (GI) were used. The presence of the putative adhesin gene, the cagA gene, and the strain specific banding pattern obtained by arbitrary primed (AP-) PCR was analyzed. Genomic fingerprinting showed extensive interpatient variation, but the banding patterns obtained from colonies from the same patient were always identical (intrapatient variation). In five patients, the cagA status varied between the colonies despite identical banding patterns. Among patients in a developed country such as Sweden, the proportion with multiple-strain infection of H. pylori is low, but subclones with differing cagA status exist within the strain.
本研究的目的是基于基因组指纹图谱和cagA（细胞毒素相关基因A）状态来确定幽门螺杆菌菌株在患者间和患者内的变异情况。研究使用了来自10例胃癌（GC）患者、10例十二指肠溃疡（DU）患者和10例胃炎（GI）患者的10个细菌菌落。分析了假定粘附素基因、cagA基因的存在情况以及通过任意引物（AP-）PCR获得的菌株特异性条带模式。基因组指纹图谱显示患者间存在广泛变异，但从同一患者的菌落获得的条带模式始终相同（患者内变异）。在5例患者中，尽管条带模式相同，但菌落之间的cagA状态有所不同。在瑞典这样的发达国家的患者中，幽门螺杆菌多重菌株感染的比例较低，但菌株内存在cagA状态不同的亚克隆。
