Hazell S L, Andrews R H, Mitchell H M, Daskalopoulous G
School of Microbiology and Immunology, University of New South Wales, Sydney, Australia.
FEMS Microbiol Lett. 1997 May 1;150(1):27-32. doi: 10.1111/j.1574-6968.1997.tb10345.x.
We investigated the population genetics of 23 isolates of H. pylori by allozyme electrophoresis using 16 enzyme loci. Isolates were obtained from adult patients of whom 48% were of Greek extraction. Eight patients (35%) had an active duodenal ulcer. Allelic variation per loci ranged from 2 to 11 alleles. Four major genetic clusters were apparent, having > 75% fixed genetic differences. There was no distinct clustering (clonal structure) on the basis of the geographical origin of the persons from whom isolates were obtained, indicating that this bacterium has not recently jumped a species barrier into humans. Isolates associated with ulcer disease were not monophyletic, with isolates from ulcer patients being found in phylogenetically diverse branches of the dendogram derived from the data. Based on the genetic diversity of H. pylori isolates, we propose that isolates should be classified as belonging not to a single species but to a 'Helicobacter pylori species-complex'.
我们通过使用16个酶位点的等位酶电泳研究了23株幽门螺杆菌的群体遗传学。分离株取自成年患者，其中48%有希腊血统。8名患者（35%）患有活动性十二指肠溃疡。每个位点的等位基因变异范围为2至11个等位基因。明显存在四个主要的遗传簇，其固定遗传差异>75%。根据分离株来源人群的地理起源，没有明显的聚类（克隆结构），这表明该细菌最近没有跨越物种屏障进入人类。与溃疡病相关的分离株并非单系的，溃疡患者的分离株出现在根据数据得出的系统发育树的不同分支中。基于幽门螺杆菌分离株的遗传多样性，我们建议将分离株分类为不属于单一物种，而是属于“幽门螺杆菌物种复合体”。
