Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome.
作者信息
Griffin D K, Haberman F, Masabanda J, O'Brien P, Bagga M, Sazanov A, Smith J, Burt D W, Ferguson-Smith M, Wienberg J
机构信息
Department of Biological Sciences, Brunel University, Uxbridge, Middlesex, UK.
出版信息
Cytogenet Cell Genet. 1999;87(3-4):278-81. doi: 10.1159/000015449.
PMID:10702695
Abstract
Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.
摘要
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