Grützner F, Zend-Ajusch E, Stout K, Munsche S, Niveleau A, Nanda I, Schmid M, Haaf T
Max Planck Institute of Molecular Genetics, Berlin, Germany.
Cytogenet Cell Genet. 2001;93(3-4):265-9. doi: 10.1159/000056996.
Microdissection of single chicken microchromosomes (MICs) followed by degenerate oligonucleotide-primed (DOP) PCR allows the rapid generation of MIC-specific DNA libraries. Since some libraries derived from a single (or a few) chromosome(s) label the entire MIC fraction, the majority of chicken MICs share repetitive DNA sequences that are not found on the macrochromosomes. In evolutionarily distant bird species, MICs are invariably hypermethylated. Methylcytosine staining provides additional in situ evidence for the high gene content of MICs and strong compartmentalization of avian genomes.
对单个鸡微小染色体（MIC）进行显微切割，随后进行简并寡核苷酸引物（DOP）PCR，能够快速构建MIC特异性DNA文库。由于一些源自单个（或少数几个）染色体的文库标记了整个MIC部分，大多数鸡的MIC共享在常染色体上未发现的重复DNA序列。在进化关系较远的鸟类物种中，MIC总是高度甲基化的。甲基胞嘧啶染色为MIC的高基因含量和鸟类基因组的强烈分区提供了额外的原位证据。
