Kataev S S, Smirnova I Iu, Zalesova V A, Kurdina L N
Sud Med Ekspert. 2000 Jan-Feb;43(1):27-32.
A chromato-mass-spectrometric method for identification of cannabinoids and 9-carboxy-11-nor-delta 9-tetrahydrocannabinol by their methyl derivatives is proposed. Use of alkaline hydrolysis in pretreatment of samples ensures the optimal conditions for complete isolation of 9-carboxy-11-nor-delta 9-tetrahydrocannabinol. Derivation with methyl iodide in absolute acetone in the presence of potassium carbonate was used. Preliminary conclusion on the presence of cannabinoids in the urine can be made from the results of enzyme immunoassay.
提出了一种通过大麻素及其甲基衍生物鉴定大麻素和9-羧基-11-去甲-Δ⁹-四氢大麻酚的色谱-质谱法。在样品预处理中使用碱性水解可确保完全分离9-羧基-11-去甲-Δ⁹-四氢大麻酚的最佳条件。采用在碳酸钾存在下于无水丙酮中用碘甲烷进行衍生化。可根据酶免疫分析结果对尿液中大麻素的存在做出初步结论。
