Vannecke C, Bloomfield M S, Vander Heyden Y, Massart D L
ChemoAc, Vrije Universiteit Brussel, Pharmaceutical Institute, Brussels, Belgium.
J Pharm Biomed Anal. 1999 Nov;21(2):241-55. doi: 10.1016/s0731-7085(99)00132-6.
A flow injection analysis (FIA) method to determine L-N-monomethylarginine, based on the reaction with ortho-phthalaldehyde in the presence of a suitable thiol-group, was optimised using experimental design. Two different approaches were followed wherein, (i) critical factors were identified in a screening design, and (ii) the simplex algorithm was used for further optimisation. In the first approach, the chemical reaction was optimised off-line and the optimal chemical conditions were transferred to the FIA-system. In the second approach the reaction and the FIA-system parameters were optimised together. The on-line approach is preferred.
一种基于在合适的硫醇基团存在下与邻苯二甲醛反应来测定L-N-单甲基精氨酸的流动注射分析（FIA）方法，通过实验设计进行了优化。采用了两种不同的方法，其中，（i）在筛选设计中确定关键因素，（ii）使用单纯形算法进行进一步优化。在第一种方法中，化学反应离线优化，然后将最佳化学条件转移到FIA系统。在第二种方法中，反应和FIA系统参数一起优化。在线方法更可取。
