Martín-Biosca Y, Sagrado S, Villaneuva-Camañas R M, Medina-Hernández M J
Departamento de Química Analítica, Universitat de València, Burjassot, Spain.
J Pharm Biomed Anal. 1999 Nov;21(2):331-8. doi: 10.1016/s0731-7085(99)00147-8.
A liquid chromatographic procedure for the determination of six barbiturates (barbital, diallyl barbituric acid, phenobarbital, butabarbital, amobarbital and pentobarbital) in urine samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C18 analytical column and a guard column of similar characteristics. The UV detector was set at 240 nm. A study to select adequate composition of the micellar mobile phase for the separation of these compounds in urine samples is performed. Maximum resolution was achieved with a 0.07 M sodium dodecylsulphate-0.3% propanol at pH 7.4 eluent. Limits of detection at 240 nm were ranged between 0.13 microg ml(-1) for diallyl barbituric acid and 2.7 microg ml(-1) for amobarbital. The procedure allows for the determination of these compounds in 20 minutes, it does not require prior a sample preparation step and it can be very useful to the investigation of intoxication.
本文描述了一种用于测定尿液样本中六种巴比妥类药物（巴比妥、二烯丙基巴比妥酸、苯巴比妥、布他比妥、异戊巴比妥和戊巴比妥）的液相色谱法。所提出的系统使用Spherisorb十八烷基硅烷ODS - 2 C18分析柱和具有相似特性的保护柱。紫外检测器设置在240 nm。进行了一项研究，以选择用于分离尿液样本中这些化合物的胶束流动相的合适组成。使用pH 7.4的0.07 M十二烷基硫酸钠 - 0.3%丙醇洗脱液可实现最大分离度。在240 nm处的检测限在二烯丙基巴比妥酸的0.13 μg ml⁻¹至异戊巴比妥的2.7 μg ml⁻¹之间。该方法可在20分钟内测定这些化合物，无需事先进行样品制备步骤，对中毒调查非常有用。
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