Martín-Biosca Y, Sagrado S, Villanueva-Camãnas R M, Medina-Hernández M J
Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, C/ Vicente A. Estellés s/n, E-46100 Burjassot, València, Spain.
Biomed Chromatogr. 2000 Apr;14(2):113-7. doi: 10.1002/(SICI)1099-0801(200004)14:2<113::AID-BMC935>3.0.CO;2-5.
A new liquid chromatographic procedure for the determination of phenobarbital in plasma samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C(18) analytical column, a guard column of similar characteristics, and a 0.03 M CTAB-3% 1-propanol at pH 7 mobile phase. The UV detector was set at 250 nm. Butabarbital was used as internal standard. Sample preparation only required the addition to the plasma samples of a 0.1 M SDS solution at pH 3 and centrifugation before injection into the chromatographic system. The limit of detection was 0.83 microg/mL of phenobarbital in plasma samples. The coefficients of variation were lower than 7. 5%.
本文描述了一种用于测定血浆样品中苯巴比妥的新型液相色谱方法。该系统使用Spherisorb十八烷基硅烷ODS-2 C(18)分析柱、具有相似特性的保护柱以及pH值为7的0.03 M CTAB - 3% 1 - 丙醇流动相。紫外检测器设置在250 nm。丁巴比妥用作内标。样品制备仅需向血浆样品中加入pH值为3的0.1 M SDS溶液，然后离心，再注入色谱系统。血浆样品中苯巴比妥的检测限为0.83微克/毫升。变异系数低于7.5%。
