Pocci R, Dixit V, Dixit V M
Varian Sample Preparation Products, Harbor City, California 90710.
J Anal Toxicol. 1992 Jan-Feb;16(1):45-7. doi: 10.1093/jat/16.1.45.
A highly selective and sensitive procedure has been developed for isolating and identifying barbiturates in human urine. With a new disposable bonded silica gel solid-phase extraction (SPE) column and hexobarbital as an internal standard (IS), amobarbital, butabarbital, pentobarbital, phenobarbital, secobarbital, and methaqualone were selectively isolated from endogenous urine components. Capillary gas chromatography/ion trap mass spectrometry (GC/MS) analysis of the extracts generated a full mass spectrum for the detection, identification, and quantitation of barbiturates. Linear quantitative response curves for the drugs have been generated over a concentration range of 20-500 ng/mL. Overall extraction efficiencies for drugs averaged greater than 90%, and the quantitative response curves exhibited correlation coefficients of 0.996 to 0.999.
已开发出一种高选择性和高灵敏度的方法，用于在人尿中分离和鉴定巴比妥类药物。使用新型一次性键合硅胶固相萃取（SPE）柱，并以己巴比妥作为内标（IS），从内源性尿液成分中选择性地分离出异戊巴比妥、仲丁巴比妥、戊巴比妥、苯巴比妥、司可巴比妥和甲喹酮。提取物的毛细管气相色谱/离子阱质谱（GC/MS）分析产生了用于巴比妥类药物检测、鉴定和定量的全质谱图。药物在20 - 500 ng/mL的浓度范围内生成了线性定量响应曲线。药物的总体提取效率平均大于90%，定量响应曲线的相关系数为0.996至0.999。
