Shamma A, Doki Y, Tsujinaka T, Shiozaki H, Inoue M, Yano M, Kawanishi K, Monden M
Department of Surgery II, Medical School, Osaka University, Osaka, Japan.
Oncology. 2000 Feb;58(2):152-8. doi: 10.1159/000012092.
Immunohistochemical studies of cell cycle-regulating proteins were conducted on tissue samples from 106 patients with esophageal squamous cell carcinoma. Reduction of p27(KIP1) was observed in 41 (39%) cases and was significantly associated with a poor prognosis by univariate and multivariate analyses (p = 0.015). In contrast, p16(INK4) expression was reduced in 55 (52%) of the cases and was not correlated with prognosis. p27(KIP1) was not correlated with the PCNA index, while p16(INK4) expression was inversely correlated with the PCNA index (p = 0.01). The expression of these two proteins was not correlated with the clinicopathological parameters of the patients. Nodal status was shown by univariate analysis (p = 0.01) to be a prognostic factor, and poorly differentiated tumors were significantly associated with a poor prognosis by multivariate analysis (p = 0.027). Thus, reduction of p27(KIP1) plays an important role in the progression of esophageal squamous cell carcinomas and is considered to be an independent prognostic indicator of this disease.
对106例食管鳞状细胞癌患者的组织样本进行了细胞周期调节蛋白的免疫组织化学研究。41例（39%）病例观察到p27（KIP1）减少，单因素和多因素分析显示其与预后不良显著相关（p = 0.015）。相比之下，55例（52%）病例p16（INK4）表达降低，且与预后无关。p27（KIP1）与增殖细胞核抗原（PCNA）指数无关，而p16（INK4）表达与PCNA指数呈负相关（p = 0.01）。这两种蛋白的表达与患者的临床病理参数无关。单因素分析显示淋巴结状态是一个预后因素（p = 0.01），多因素分析显示低分化肿瘤与预后不良显著相关（p = 0.027）。因此，p27（KIP1）减少在食管鳞状细胞癌进展中起重要作用，被认为是该疾病的一个独立预后指标。
