Albuerne M, De Lavallina J, Esteban I, Naves F J, Silos-Santiago I, Vega J A
Departamento de Morfología y Biología Celular, Universidad de Oviedo, 33006 Oviedo, Spain.
Anat Rec. 2000 Mar 1;258(3):235-42. doi: 10.1002/(SICI)1097-0185(20000301)258:3<235::AID-AR2>3.0.CO;2-M.
The development of Meissner-like and Pacinian corpuscles was studied in mice [from postnatal day (Pd) 0 to 42] by using immunohistochemistry for specific corpuscular constituents. The battery of antigens investigated included PGP 9.5 protein and neurofilaments, as markers for the central axon; S100 protein, vimentin, and p75(LNGFR) protein, to show Schwann-related cells; and epithelial membrane antigen to identify perineurial-related cells. In Meissner-like corpuscles immunoreactivity (IR) for neuronal markers was found by Pd7 and later. The lamellar cells of these corpuscles expressed first S100 protein IR (Pd7 to Pd42), then vimentin IR (Pd12 to Pd42), and transitory p75(LNGFR) IR (Pd7 to Pd19-20). Vimentin IR, but not epithelial membrane antigen, was detected in the capsule-like cells of the Meissner-like corpuscles. On the other hand, the density of Meissner-like corpuscles progressively increased from Pd0 to Pd19-20. Pacinian corpuscles were identified by Pd7. From this time to Pd42 the central axon showed IR for neuronal markers, and the inner core cells were immunoreactive for S100 protein. Moreover, vimentin IR was detected in the inner core cells by Pd19 and later. Unexpectedly, the central axons displayed S100 protein IR (from Pd7 to P28), while p75(LNGFR) protein IR or epithelial membrane antigen IR were never detected. Taken together, and based on the expression of the assessed antigens alone, the present results suggest that the Meissner-like and the Pacinian corpuscles in mice become mature around Pd19-Pd28 and Pd20, respectively. Furthermore, these results provide a baseline timetable for future studies in the normal or altered development of sensory corpuscles in mice since specific sensory corpuscles are functionally associated with different subtypes of sensory neurons the development of which is selectively disturbed in genetically manipulated mice.
通过对特定小体成分进行免疫组织化学分析，研究了小鼠（从出生后第0天到第42天）类梅斯纳小体和帕西尼小体的发育情况。所研究的一系列抗原包括作为中枢轴突标志物的PGP 9.5蛋白和神经丝；用于显示雪旺氏相关细胞的S100蛋白、波形蛋白和p75（LNGFR）蛋白；以及用于识别神经束膜相关细胞的上皮膜抗原。在类梅斯纳小体中，到出生后第7天及之后发现了神经元标志物的免疫反应性（IR）。这些小体的板层细胞首先表达S100蛋白IR（出生后第7天到第42天），然后是波形蛋白IR（出生后第12天到第42天），以及短暂的p75（LNGFR）IR（出生后第7天到第19 - 20天）。在类梅斯纳小体的囊样细胞中检测到波形蛋白IR，但未检测到上皮膜抗原。另一方面，类梅斯纳小体的密度从出生后第0天到第19 - 20天逐渐增加。帕西尼小体在出生后第7天被识别出来。从此时到出生后第42天，中枢轴突显示出神经元标志物的IR，内核细胞对S100蛋白具有免疫反应性。此外，到出生后第19天及之后在内核细胞中检测到波形蛋白IR。出乎意料的是，中枢轴突显示出S100蛋白IR（从出生后第7天到第28天），而从未检测到p75（LNGFR）蛋白IR或上皮膜抗原IR。综上所述，仅基于所评估抗原的表达，目前的结果表明，小鼠中的类梅斯纳小体和帕西尼小体分别在出生后第19 - 28天和第20天左右成熟。此外，这些结果为未来研究小鼠感觉小体正常或改变的发育提供了一个基线时间表，因为特定的感觉小体在功能上与不同亚型的感觉神经元相关，而在基因操作小鼠中这些感觉神经元的发育会受到选择性干扰。
