端粒酶检测在口腔恶性肿瘤患者淋巴结转移检测中的临床应用价值。
Clinical usefulness of telomerase assay for the detection of lymph node metastasis in patients with oral malignancy.
作者信息
Sumida T, Hamakawa H, Kayahara H, Zen H, Sogawa K, Tanioka H, Ueda N
机构信息
Department of Oral Surgery, Ehime University School of Medicine, Japan.
出版信息
Arch Pathol Lab Med. 2000 Mar;124(3):398-400. doi: 10.5858/2000-124-0398-CUOTAF.
OBJECTIVE
Telomerase is considered a diagnostic marker of malignancy. We investigated the usefulness of telomerase assay for the detection of lymph node micrometastasis.
METHODS
Sixteen cervical lymph nodes with metastasis of oral cancer and 20 benign lymph nodes were studied. The oral cancer cell line was used to estimate the sensitivity for telomerase assay. Telomerase activity was measured by semiquantitative telomeric repeat amplification protocol.
RESULTS
There was a significant difference between malignant and benign lymph nodes. The telomerase activity of 50 mg of lymph nodes with 103 or more cancer cells differed from that of control lymph nodes. Lymph nodes with 102 or fewer tumor cells expressed similar levels as benign lymph nodes.
CONCLUSIONS
In addition to routine histologic examination, telomerase assay is considered a useful tool for the detection of lymph node metastasis in patients with oral malignancy.
目的
端粒酶被认为是恶性肿瘤的诊断标志物。我们研究了端粒酶检测在检测淋巴结微转移中的实用性。
方法
研究了16个发生口腔癌转移的颈部淋巴结和20个良性淋巴结。使用口腔癌细胞系评估端粒酶检测的敏感性。通过半定量端粒重复序列扩增法测量端粒酶活性。
结果
恶性和良性淋巴结之间存在显著差异。含有103个或更多癌细胞的50毫克淋巴结的端粒酶活性与对照淋巴结不同。含有102个或更少肿瘤细胞的淋巴结表达水平与良性淋巴结相似。
结论
除常规组织学检查外，端粒酶检测被认为是检测口腔恶性肿瘤患者淋巴结转移的有用工具。
Zotero 插件