Liu G, Li J, Li Z, Chen X
College of Stomatology, Wuhan University.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2001 Aug;19(4):216-8, 224.
This study is aim at investigating the relationship between the expression and activity of MMP-2,9, TIMP-1,2 and the metastasis of oral squamous cell carcinoma to neck lymph node.
The expression of MMP-2,9, TIMP-1,2 in 30 oral squamous cell carcinoma, two oral squamous cell carcinoma cell lines GNM and TSCCa was detected by in situ hybridization; Zomography was performed to determined the activity of MMP-2,9 in cancer tissue and conditioned medium of GNM and TSCCa; Ability of invasion of GNM and TSCCa was measured with in vitro invasion assays.
Both tumor cells and stroma expressed both MMP-2,9 and TIMP-1,2; It was found that MMP-2,9, TIMP-1,2 had a higher expression in patients with neck lymph node metastasis than those without metastasis (P < 0.05), positive percentage of MMP-2,9 in GNM was higher than that in TSCCa; The MMP-2,9 in node-positive patients was more active than in node-negative ones (P < 0.05); GNM had more active MMP-2, 9 and higher invasive potential than TSCCa.
MMP-2,9 may play an important role in metastasis of oral squamous cell carcinoma; Elevation of TIMP-1,2 is likely to be the result of interaction of tumor and stroma.
本研究旨在探讨基质金属蛋白酶-2、9（MMP-2、9）及金属蛋白酶组织抑制因子-1、2（TIMP-1、2）的表达及活性与口腔鳞状细胞癌颈部淋巴结转移之间的关系。
采用原位杂交技术检测30例口腔鳞状细胞癌组织及2种口腔鳞状细胞癌细胞系GNM和TSCCa中MMP-2、9及TIMP-1、2的表达；运用酶谱分析法检测癌组织及GNM和TSCCa条件培养基中MMP-2、9的活性；通过体外侵袭实验检测GNM和TSCCa的侵袭能力。
肿瘤细胞和基质中均有MMP-2、9及TIMP-1、2的表达；发现颈部淋巴结转移患者的MMP-2、9及TIMP-1、2表达高于无转移患者（P<0.05），GNM中MMP-2、9的阳性率高于TSCCa；有淋巴结转移患者的MMP-2、9活性高于无转移者（P<0.05）；GNM的MMP-2、9活性更强，侵袭潜能高于TSCCa。
MMP-2、9可能在口腔鳞状细胞癌转移中起重要作用；TIMP-1、2升高可能是肿瘤与基质相互作用的结果。
