Shuruta S A, Amerkhanov Z G, Postnikova G B
Institute of Cell Biophysics, Russian Academy of Sciences, Moscow Region, Russia.
Biofizika. 1999 Nov-Dec;44(6):1059-62.
The effect of mitochondria on the redox reaction between oxymyoglobin (oxy-Mb) and ferricytochrome c was studied. The parameters of this reaction in the absence of mitochondria have been investigated earlier. It is shown that the course of oxidation of oxymyoglobin by cytochrome c in the presence of mitochondria differs from that without mitochondria: no reduced cytochrome c is observed; in addition, the order of this redox reaction and its dependence on pH and ionic strength change. The factors influencing the state of mitochondrial membrane and uncouples enhance markedly the reaction rate. The conclusion was drawn that mitochondria directly participate in the oxymyoglobin-cytochrome c redox reaction. The possibility of this reaction in vivo under extreme conditions and during pathological processes is discussed.
研究了线粒体对氧合肌红蛋白（oxy-Mb）与高铁细胞色素c之间氧化还原反应的影响。此前已对无线粒体时该反应的参数进行了研究。结果表明，存在线粒体时细胞色素c氧化氧合肌红蛋白的过程与不存在线粒体时不同：未观察到还原型细胞色素c；此外，该氧化还原反应的顺序及其对pH和离子强度的依赖性发生了变化。影响线粒体膜状态的因素和解偶联剂显著提高了反应速率。得出的结论是线粒体直接参与了氧合肌红蛋白-细胞色素c氧化还原反应。讨论了在极端条件下和病理过程中该反应在体内发生的可能性。
