Starodubtseva M N, Ignatenko V A, Cherenkevich S N
Gomel State Medical Institute, Belarus.
Biofizika. 1999 Nov-Dec;44(6):1068-72.
The formation of two hemoglobin forms (methemoglobin and nitrite methemoglobin) in native human erythrocytes in the presence of sodium nitrite in suspension was shown. In normal erythrocytes, the interaction of intracellular oxyhemoglobin with nitrite ions results in the formation of methemoglobin, whereas in metabolically exhausted erythrocytes, this leads predominantly to the formation of nitrite methemoglobin. The nitrite methemoglobin reacts with hydrogen peroxide to form reactive intermediates (e.g. peroxynitrous acid) and the products of hemoglobin destruction. During the storage of erythrocyte suspensions containing methemoglobin and modified nitrite methemoglobin, differences in the forms of erythrocytes and the degree of their hemolysis were revealed. It is assumed that the formation of methemoglobin leads to the destruction of erythrocytes.
结果表明，在悬浮状态下亚硝酸钠存在时，天然人红细胞中会形成两种血红蛋白形式（高铁血红蛋白和亚硝酸高铁血红蛋白）。在正常红细胞中，细胞内氧合血红蛋白与亚硝酸根离子相互作用会导致高铁血红蛋白的形成，而在代谢耗尽的红细胞中，这主要导致亚硝酸高铁血红蛋白的形成。亚硝酸高铁血红蛋白与过氧化氢反应形成反应性中间体（如过氧亚硝酸）和血红蛋白破坏产物。在储存含有高铁血红蛋白和修饰的亚硝酸高铁血红蛋白的红细胞悬液过程中，发现了红细胞形式及其溶血程度的差异。据推测，高铁血红蛋白的形成会导致红细胞的破坏。
