Smith R C, Nunn V
Arch Biochem Biophys. 1984 Jul;232(1):348-53. doi: 10.1016/0003-9861(84)90550-2.
The addition of sodium nitrite to washed bovine erythrocytes incubated in buffered saline resulted in the formation of methemoglobin with a decrease in the concentration of 3-N-ribosyluric acid. The oxyhemoglobin in hemolysates prepared from bovine red cells which contained high concentrations of 3-ribosyluric acid was oxidized to methemoglobin more slowly than oxyhemoglobin from cells with low levels of 3-ribosyluric acid. Oxyhemoglobin from hemolysates that were dialyzed was oxidized more rapidly than oxyhemoglobin which was not dialyzed. 3-Ribosyluric acid, glutathione, uric acid, and ascorbic acid prevented the oxidation of oxyhemoglobin by nitrite. Uric acid protected oxyhemoglobin at the lowest concentration, followed closely by ascorbic acid, 3-ribosyluric acid, and glutathione. Hydrogen peroxide enhanced the oxidation produced by nitrite; this effect was also prevented by the four antioxidants used.
向在缓冲盐溶液中孵育的洗涤过的牛红细胞中添加亚硝酸钠，会导致高铁血红蛋白的形成，同时3 - N - 核糖基尿酸的浓度降低。从含有高浓度3 - 核糖基尿酸的牛红细胞制备的溶血产物中的氧合血红蛋白，被氧化为高铁血红蛋白的速度比来自低水平3 - 核糖基尿酸细胞的氧合血红蛋白慢。经过透析的溶血产物中的氧合血红蛋白比未透析的氧合血红蛋白被氧化得更快。3 - 核糖基尿酸、谷胱甘肽、尿酸和抗坏血酸可防止亚硝酸盐氧化氧合血红蛋白。尿酸在最低浓度时就能保护氧合血红蛋白，其次是抗坏血酸、3 - 核糖基尿酸和谷胱甘肽。过氧化氢会增强亚硝酸盐产生的氧化作用；这一作用也被所使用的四种抗氧化剂所阻止。
