Huang M L, Hsu C H, Chien C T
Institute of Neuroscience, National Yang-Ming University, Taipei, Taiwan.
Neuron. 2000 Jan;25(1):57-67. doi: 10.1016/s0896-6273(00)80871-5.
In the Drosophila peripheral nervous system, proneural genes direct the formation of different types of sensory organs. Here, we show that amos is a novel proneural gene that promotes multiple dendritic (MD) neuron formation. amos encodes a basic-helix-loop-helix (bHLH) protein of the Atonal family. During embryonic development, amos is expressed in patches of ectodermal cells, and the expression is quickly restricted to sensory organ precursors. Loss of amos function eliminates MD neurons that remain in ASC;atonal mutants. Misexpression of amos generates ectopic MD and other types of neurons. Amos interacts with the ubiquitously expressed Daughter-less protein in vivo and in vitro. Our final misexpression experiments suggest that a domain located outside the DNA-binding domain of Amos determines the MD neuronal specificity.
在果蝇外周神经系统中，原神经基因指导不同类型感觉器官的形成。在此，我们表明阿莫斯（amos）是一个促进多树突（MD）神经元形成的新型原神经基因。阿莫斯编码一种无调性家族的碱性螺旋-环-螺旋（bHLH）蛋白。在胚胎发育过程中，阿莫斯在外胚层细胞斑块中表达，且该表达迅速局限于感觉器官前体。阿莫斯功能缺失会消除在无调性突变体的ASC中残留的MD神经元。阿莫斯的错误表达会产生异位MD神经元和其他类型的神经元。阿莫斯在体内和体外均与普遍表达的无女儿（Daughter-less）蛋白相互作用。我们最后的错误表达实验表明，位于阿莫斯DNA结合结构域之外的一个结构域决定了MD神经元的特异性。
