Chye S M, Chang J H, Yen C M
Fooyin Institute of Technology, Taiwan, ROC.
Acta Trop. 2000 Feb 25;75(1):9-17. doi: 10.1016/s0001-706x(99)00082-0.
An antigen from Angiostrongylus cantonensis fifth-stage larvae was purified by immuno-affinity chromatography with a specific monoclonal antibody. The purified antigen showed only a single band with a molecular weight of 204 kD in SDS-PAGE, and no cross-reactivity to antibodies induced by several other species of helminths were observed in ELISA. When the purified antigen was used to examine serum and cerebrospinal fluid (CSF) specimens by ELISA, the antibody levels in patients with eosinophilic meningitis or meningoencephalitis (EME) were significantly higher than those of control subjects. The antibody levels in serum were slightly higher than those in CSF, and the levels in serum were positively correlated with the levels in CSF. The reliability in detection of antibodies in serum was slightly higher than that in the detection of antibodies in CSF specimens. The purification of a specific A. cantonensis antigen and its subsequent use in the development of an ELISA for detection of A. cantonensis specific antibodies in serum specimens constitute an important step towards improvement in the accuracy of diagnosis for A. cantonensis infections.
用特异性单克隆抗体通过免疫亲和层析法纯化广州管圆线虫第五期幼虫的一种抗原。纯化后的抗原在SDS - PAGE中仅显示一条分子量为204 kD的条带，且在ELISA中未观察到与其他几种蠕虫诱导产生的抗体发生交叉反应。当用纯化后的抗原通过ELISA检测血清和脑脊液（CSF）标本时，嗜酸性粒细胞性脑膜炎或脑膜脑炎（EME）患者的抗体水平显著高于对照受试者。血清中的抗体水平略高于脑脊液中的抗体水平，且血清中的水平与脑脊液中的水平呈正相关。血清中抗体检测的可靠性略高于脑脊液标本中抗体检测的可靠性。纯化一种特异性广州管圆线虫抗原并随后将其用于开发ELISA以检测血清标本中广州管圆线虫特异性抗体，是提高广州管圆线虫感染诊断准确性的重要一步。
