Yang Y, Wilson D L
Department of Medicine, University of Miami School of Medicine, FL 33101, USA.
Mech Ageing Dev. 2000 Feb 7;113(2):101-16. doi: 10.1016/s0047-6374(99)00100-1.
We designed a novel procedure for the isolation of mutant strains with significantly increased life spans in the nematode Caenorhabditis elegans. This procedure involves using heat-shock to screen a large number of animals and isolate a few which are more resistant to heat-shock stress. From the heat-shock-resistant animals, three mutant strains, HG25, HG96, and HG246, all exhibiting increased life span, were isolated. One mutant strain (HG246) develops more slowly than the wild-type strain, N2. Two mutant strains, HG96 and HG246, exhibit lower fertility than the wild-type. Each of the three mutant strains has a normal appearance. Their locomotive behavior also appears normal; only HG246 shows slightly slower movement. Their feeding behavior appears normal, and the males of HG25 and HG96 show normal mating behavior. However, the males of HG246, either are defective in their mating ability or their sperm are defective. The results indicate that heat-shock can be used as a means to facilitate the isolation of mutants which have longer life expectancy.
我们设计了一种全新的程序，用于在秀丽隐杆线虫中分离寿命显著延长的突变菌株。该程序包括利用热休克筛选大量线虫，并分离出少数对热休克应激更具抗性的线虫。从抗热休克的线虫中，分离出了三个寿命均有所延长的突变菌株，即HG25、HG96和HG246。其中一个突变菌株（HG246）的发育速度比野生型菌株N2慢。两个突变菌株HG96和HG246的繁殖力低于野生型。这三个突变菌株的外观均正常。它们的运动行为看起来也正常；只有HG246的运动略显迟缓。它们的进食行为看起来正常，HG25和HG96的雄性表现出正常的交配行为。然而，HG246的雄性要么交配能力有缺陷，要么精子有缺陷。结果表明，热休克可作为一种手段，便于分离预期寿命更长的突变体。
