Atalay F, Baltaci V, Alpas I, Savas I, Atikcan S, Balci S
Atatürk Chest Diseases and Chest Surgery Center, Ankara, Turkey.
Mutat Res. 2000 Feb 16;465(1-2):159-63. doi: 10.1016/s1383-5718(99)00224-7.
This study assessed the impact of malignant mesothelioma on the frequencies of sister chromatid exchange (SCE) in the pleural effusion cells. Ten patients with mesothelioma and 20 control subjects were included in the study. The control subjects were the patients with tuberculosis pleurisy, and the remaining 10 subjects of control group were healthy volunteers and only heparinized blood samples were collected from these subjects. The pleural effusion cells were cultured with conventional culture methods. The samples were obtained from the patients after histopathologic confirmation of the malignancy but before the initiation of chemotherapy or radiotherapy. At the end of the culture period and 48 h prior the harvesting, BrdU was added into flasks. Totally, 100 metaphases were scored for each sample. In this study, we found that the SCE frequencies of malignant pleural mesotheliomas were significantly higher than the control subjects (P<0.001). Six of 10 patients came from central Anatolia, which is of great importance due to high rate of exposure to asbestosis in this region.
本研究评估了恶性间皮瘤对胸腔积液细胞中姐妹染色单体交换（SCE）频率的影响。该研究纳入了10例间皮瘤患者和20名对照对象。对照对象为结核性胸膜炎患者，对照组其余10名对象为健康志愿者，仅从这些对象采集了肝素化血样。采用常规培养方法培养胸腔积液细胞。样本是在恶性肿瘤经组织病理学确诊后、化疗或放疗开始前从患者身上获取的。在培养期结束时及收获前48小时，向培养瓶中加入溴脱氧尿苷（BrdU）。每个样本共计数100个中期分裂相。在本研究中，我们发现恶性胸膜间皮瘤的SCE频率显著高于对照对象（P<0.001）。10例患者中有6例来自安纳托利亚中部，该地区因石棉沉着病暴露率高而具有重要意义。
