Kageyama A, Benno Y
Japan Collection of Microorganisms, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama.
Microbiol Immunol. 2000;44(1):23-8. doi: 10.1111/j.1348-0421.2000.tb01242.x.
Three strains of Eubacterium-like isolates from human feces were characterized by biochemical tests and 16S rDNA analysis. The phenotypic characteristics of the three strains resembled those of the genus Collinsella transferred from the genus Eubacterium recently. However, Eubacterium-like strains were phylogenetically members of the Clostridium subphylum of gram-positive bacteria, and these showed a specific phylogenetic association with Clostridium ramosum and C. spiroforme. C. ramosum and C. spiroforme are gram-positive, anaerobic, spore-forming bacteria that belong to the genus Clostridium, and the G + C contents are 26.0 and 27.4 mol%, respectively. However, the three Eubacterium-like strains had G + C contents of 32.1 to 33.1 mol% and were non-spore-forming rods. Based on phenotypic characteristics, we can differentiate these species, and furthermore, a 16S rDNA sequence divergence of greater than 9% with a new related genus, Coprobacillus, is proposed for the three strains, with one species, Coprobacillus catenaformis. The type strain of C. catenaformis is JCM 10604T.
通过生化试验和16S rDNA分析对三株从人粪便中分离出的类真杆菌菌株进行了鉴定。这三株菌株的表型特征与最近从真杆菌属转移过来的柯林斯菌属相似。然而，类真杆菌菌株在系统发育上属于革兰氏阳性菌梭菌亚门成员，并且与多枝梭菌和螺旋形梭菌表现出特定的系统发育关联。多枝梭菌和螺旋形梭菌是革兰氏阳性、厌氧、产芽孢细菌，属于梭菌属，其G+C含量分别为26.0和27.4mol%。然而，这三株类真杆菌菌株的G+C含量为32.1至33.1mol%，为无芽孢杆菌。基于表型特征，我们可以区分这些菌种，此外，这三株菌株与一个新的相关属——粪芽孢杆菌属的16S rDNA序列差异大于9%，其中一个种为链状粪芽孢杆菌。链状粪芽孢杆菌的模式菌株为JCM 10604T。
