Nakazawa F, Poco S E, Ikeda T, Sato M, Kalfas S, Sundqvist G, Hoshino E
Department of Oral Microbiology, School of Dentistry, Niigata University, Japan.
Int J Syst Bacteriol. 1999 Jul;49 Pt 3:1193-200. doi: 10.1099/00207713-49-3-1193.
Novel Eubacterium-like isolates, strains 12-3T and KV43-B, which were isolated from the periodontal pocket of an adult patient with periodontal disease and necrotic dental pulp, respectively, were studied taxonomically and phylogenetically. The morphological and differential biochemical characteristics of these organisms are also described in this paper. These organisms were Gram-positive, anaerobic, non-spore-forming, rod-shaped bacteria that were inert in most of the conventional biochemical tests and closely resembled members of asaccharolytic oral Eubacterium species. On the other hand, protein profiles of whole cells in SDS-PAGE and Western immunoblotting reaction analysis distinguished these isolates from strains of the previously described genus Eubacterium. The G+C content of the DNAs from the novel isolates was 50 and 51 mol%, respectively. The levels of DNA-DNA relatedness to other asaccharolytic oral Eubacterium species, including Eubacterium brachy, Eubacterium lentum, Eubacterium nodatum, Eubacterium timidum, Eubacterium saphenum, Eubacterium minutum and Eubacterium exiguum, was less than 11%. These organisms also exhibited a very low level of reassociation with the DNA of Eubacterium limosum, the type species of the genus Eubacterium. The results of 16S rDNA sequence comparisons revealed that these organisms represent a novel lineage distinct from all previously described genera of Gram-positive, rod-shaped bacteria. On the basis of our results, it is suggested that strains 12-3T and KV43-B should be classified in a new genus and species, for which the name Cryptobacterium curtum gen. nov., sp. nov. is proposed. The type strain of Cryptobacterium curtum is 12-3T (= ATCC 700683T).
分别从一名患有牙周病和坏死牙髓的成年患者的牙周袋中分离出的新型类真杆菌分离株，即12 - 3T菌株和KV43 - B菌株，进行了分类学和系统发育学研究。本文还描述了这些微生物的形态学和鉴别生化特征。这些微生物为革兰氏阳性、厌氧、无芽孢形成、杆状细菌，在大多数常规生化试验中呈惰性，与不产糖的口腔真杆菌属成员极为相似。另一方面，十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）中全细胞的蛋白质谱以及Western免疫印迹反应分析将这些分离株与先前描述的真杆菌属菌株区分开来。新型分离株DNA的G + C含量分别为50%和51%。与其他不产糖的口腔真杆菌属物种，包括短真杆菌、迟缓真杆菌、结节真杆菌、胆小真杆菌、隐蔽真杆菌、微小真杆菌和苛求真杆菌的DNA - DNA相关性水平低于11%。这些微生物与真杆菌属模式种黏液真杆菌的DNA的重结合水平也非常低。16S rDNA序列比较结果表明，这些微生物代表了一个与先前描述的所有革兰氏阳性、杆状细菌属不同的新谱系。根据我们的结果，建议将12 - 3T菌株和KV43 - B菌株归入一个新的属和种，为此提出新属名Cryptobacterium curtum gen. nov.，新种名Cryptobacterium curtum sp. nov.。Curtum隐杆菌的模式菌株为12 - 3T（= ATCC 700683T）。
