Di Mauro E, Kendrew S G, Caserta M
Centro di Studio per gli Acidi Nucleici, Consiglio Nazionale delle Ricerche, Università "La Sapienza," P.le Aldo Moro 5, 00185 Rome, Italy.
J Biol Chem. 2000 Mar 17;275(11):7612-8. doi: 10.1074/jbc.275.11.7612.
Glucose depletion derepresses the Saccharomyces cerevisiae ADH2 gene; this metabolic change is accompanied by chromatin structural modifications in the promoter region. We show that the ADR6/SWI1 gene is not necessary for derepression of the wild type chromosomal ADH2, whereas the transcription factor Adr1p, which regulates several S. cerevisiae functions, plays a major role in driving nucleosome reconfiguration and ADH2 expression. When we tested the effect of individual domains of the regulatory protein Adr1p on the chromatin structure of ADH2, a remodeling consisting of at least two steps was observed. Adr1p derivatives were analyzed in derepressing conditions, showing that the Adr1p DNA binding domain alone causes an alteration in chromatin organization in the absence of transcription. This alteration differs from the remodeling observed in the presence of the Adr1p activation domain when the promoter is transcriptionally active.
葡萄糖耗竭可解除酿酒酵母ADH2基因的阻遏；这种代谢变化伴随着启动子区域染色质结构的修饰。我们发现，ADR6/SWI1基因对于野生型染色体ADH2的去阻遏并非必需，而调节酿酒酵母多种功能的转录因子Adr1p在驱动核小体重构和ADH2表达中起主要作用。当我们测试调节蛋白Adr1p的各个结构域对ADH2染色质结构的影响时，观察到了至少由两个步骤组成的重塑过程。在去阻遏条件下对Adr1p衍生物进行了分析，结果表明，单独的Adr1p DNA结合结构域在无转录的情况下会导致染色质组织发生改变。这种改变不同于当启动子转录活跃时在存在Adr1p激活结构域的情况下所观察到的重塑。
