枯草芽孢杆菌孢子发育所需的SecA-SecY相互作用的遗传分析。

Genetic analysis of SecA-SecY interaction required for spore development in Bacillus subtilis.

作者信息

Kobayashi H, Ohashi Y, Nanamiya H, Asai K, Kawamura F

机构信息

Laboratory of Molecular Genetics, College of Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo, Japan.

出版信息

FEMS Microbiol Lett. 2000 Mar 15;184(2):285-9. doi: 10.1111/j.1574-6968.2000.tb09028.x.

DOI:10.1111/j.1574-6968.2000.tb09028.x

PMID:10713435

Abstract

All spontaneous suppressor mutations obtained from a secA12 sporulation-defective mutant in Bacillus subtilis were localized in highly conserved membrane-spanning regions of SecY. The expression of early sporulation genes, kinA and spo0A encoding a histidine kinase and a transcription regulator for several sporulation genes, respectively, was restored in these suppressor mutants. These results indicate that the secretion function of translocase combined with Sec proteins is required for sporulation in B. subtilis.

摘要

从枯草芽孢杆菌的secA12孢子形成缺陷型突变体中获得的所有自发抑制突变都定位在SecY高度保守的跨膜区域。在这些抑制突变体中，早期孢子形成基因kinA和spo0A的表达得以恢复，kinA和spo0A分别编码一种组氨酸激酶和几种孢子形成基因的转录调节因子。这些结果表明，与Sec蛋白结合的转位酶的分泌功能是枯草芽孢杆菌孢子形成所必需的。