Asai K, Kawamura F, Sadaie Y, Takahashi H
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Japan.
J Bacteriol. 1997 Jan;179(2):544-7. doi: 10.1128/jb.179.2.544-547.1997.
A Bacillus subtilis secA mutant, secA12, which is blocked at an early stage of sporulation, is able to grow as well as the wild-type strain at all temperatures tested. Experiments with lacZ fusion genes showed that the induction of kinA expression, as well as the sporulation-specific transcription of the spo0A gene, was not observed in the secA12 mutant. However, transcription of the spo0H gene (coding for sigmaH, which is required for the transcription of kinA and spo0A) and accumulation of the sigmaH protein were not affected in secA12. These results suggested that mutations in secA affect a factor required for efficient transcription of kinA as well as for the activation of the phosphorelay pathway.
一种枯草芽孢杆菌secA突变体secA12，在芽孢形成的早期阶段受阻，在所有测试温度下都能与野生型菌株一样良好生长。利用lacZ融合基因进行的实验表明，在secA12突变体中未观察到kinA表达的诱导以及spo0A基因的芽孢形成特异性转录。然而，secA12中spo0H基因（编码kinA和spo0A转录所需的σH）的转录和σH蛋白的积累不受影响。这些结果表明，secA中的突变影响了kinA有效转录以及磷酸传递途径激活所需的一个因子。
