枯草芽孢杆菌secA基因中孢子形成起始突变的分离与鉴定
Isolation and characterization of a sporulation initiation mutation in the Bacillus subtilis secA gene.
作者信息
Asai K, Kawamura F, Sadaie Y, Takahashi H
机构信息
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Japan.
出版信息
J Bacteriol. 1997 Jan;179(2):544-7. doi: 10.1128/jb.179.2.544-547.1997.
Abstract
A Bacillus subtilis secA mutant, secA12, which is blocked at an early stage of sporulation, is able to grow as well as the wild-type strain at all temperatures tested. Experiments with lacZ fusion genes showed that the induction of kinA expression, as well as the sporulation-specific transcription of the spo0A gene, was not observed in the secA12 mutant. However, transcription of the spo0H gene (coding for sigmaH, which is required for the transcription of kinA and spo0A) and accumulation of the sigmaH protein were not affected in secA12. These results suggested that mutations in secA affect a factor required for efficient transcription of kinA as well as for the activation of the phosphorelay pathway.
摘要
一种枯草芽孢杆菌secA突变体secA12，在芽孢形成的早期阶段受阻，在所有测试温度下都能与野生型菌株一样良好生长。利用lacZ融合基因进行的实验表明，在secA12突变体中未观察到kinA表达的诱导以及spo0A基因的芽孢形成特异性转录。然而，secA12中spo0H基因（编码kinA和spo0A转录所需的σH）的转录和σH蛋白的积累不受影响。这些结果表明，secA中的突变影响了kinA有效转录以及磷酸传递途径激活所需的一个因子。
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本文引用的文献
1
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌转化的要求。
J Bacteriol. 1961 May;81(5):741-6. doi: 10.1128/jb.81.5.741-746.1961.
2
Multisensory activation of the phosphorelay initiating sporulation in Bacillus subtilis: identification and sequence of the protein kinase of the alternate pathway.枯草芽孢杆菌中启动芽孢形成的磷酸化信号转导的多感官激活：替代途径蛋白激酶的鉴定与序列分析
Mol Microbiol. 1993 Apr;8(1):69-79. doi: 10.1111/j.1365-2958.1993.tb01204.x.
3
A positive feedback loop controls transcription of the spoOF gene, a component of the sporulation phosphorelay in Bacillus subtilis.一个正反馈环控制着枯草芽孢杆菌中芽孢形成磷酸信号转导途径的一个组成部分spoOF基因的转录。
Mol Microbiol. 1993 Mar;7(6):967-74. doi: 10.1111/j.1365-2958.1993.tb01188.x.
4
Two distinct ATP-binding domains are needed to promote protein export by Escherichia coli SecA ATPase.大肠杆菌SecA ATP酶促进蛋白质输出需要两个不同的ATP结合结构域。
Mol Microbiol. 1993 Nov;10(3):483-97. doi: 10.1111/j.1365-2958.1993.tb00921.x.
5
Expression of kinA and accumulation of sigma H at the onset of sporulation in Bacillus subtilis.枯草芽孢杆菌孢子形成起始时kinA的表达及σH的积累。
J Bacteriol. 1995 Nov;177(22):6679-83. doi: 10.1128/jb.177.22.6679-6683.1995.
6
Effects of spo0 mutations on spo0A promoter switching at the initiation of sporulation in Bacillus subtilis.枯草芽孢杆菌孢子形成起始时spo0突变对spo0A启动子转换的影响。
J Bacteriol. 1995 Aug;177(15):4520-3. doi: 10.1128/jb.177.15.4520-4523.1995.
7
Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli.鉴定大肠杆菌中一个参与包膜蛋白分泌的新基因（secA）及其基因产物。
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8
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J Bacteriol. 1985 Aug;163(2):648-53. doi: 10.1128/jb.163.2.648-653.1985.
9
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J Bacteriol. 1988 Mar;170(3):1054-62. doi: 10.1128/jb.170.3.1054-1062.1988.
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Mol Microbiol. 1988 Nov;2(6):689-99. doi: 10.1111/j.1365-2958.1988.tb00079.x.
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