Raisis A L, Young L E, Taylor P M, Walsh K P, Lekeux P
Center for Equine Studies, The Animal Health Trust, Newmarket, Suffolk, UK.
Am J Vet Res. 2000 Mar;61(3):286-90. doi: 10.2460/ajvr.2000.61.286.
To use Doppler ultrasonography and single-fiber laser Doppler flowmetry (LDF) to evaluate blood flow in the dependent and nondependent hind limbs of anesthetized horses and to evaluate changes in femoral arterial blood flow and microvascular skeletal muscle perfusion in response to administration of phenylephrine hydrochloride or dobutamine hydrochloride.
6 healthy adult horses.
Horses were anesthetized and positioned in left lateral recumbency. Doppler ultrasonography was used to measure velocity and volumetric flow in the femoral vessels. Single-fiber LDF was used to measure relative microvascular perfusion at a single site in the semimembranosus muscles. Phenylephrine or dobutamine was then administered to decrease or increase femoral arterial blood flow, and changes in blood flow and microvascular perfusion were recorded.
Administration of phenylephrine resulted in significant decreases in femoral arterial and venous blood flows and cardiac output and significant increases in mean aortic blood pressure, systemic vascular resistance, and PCV. Administration of dobutamine resulted in significant increases in femoral arterial blood flow, mean aortic blood pressure, and PCV. Significant changes in microvascular perfusion were not detected.
Results suggest that Doppler ultrasonography and single-fiber LDF can be used to study blood flows in the hind limbs of anesthetized horses. However, further studies are required to determine why changes in femoral arterial blood flows were not associated with changes in microvascular perfusion.
使用多普勒超声和单纤维激光多普勒血流仪（LDF）评估麻醉马匹后肢下垂和非下垂部位的血流，并评估静脉注射盐酸去氧肾上腺素或盐酸多巴酚丁胺后股动脉血流和骨骼肌微血管灌注的变化。
6匹健康成年马。
将马匹麻醉并置于左侧卧位。使用多普勒超声测量股血管中的血流速度和容积流量。使用单纤维LDF测量半膜肌单个部位的相对微血管灌注。然后给予去氧肾上腺素或多巴酚丁胺以减少或增加股动脉血流，并记录血流和微血管灌注的变化。
给予去氧肾上腺素后，股动脉和静脉血流以及心输出量显著降低，平均主动脉血压、全身血管阻力和红细胞压积显著升高。给予多巴酚丁胺后，股动脉血流、平均主动脉血压和红细胞压积显著升高。未检测到微血管灌注的显著变化。
结果表明，多普勒超声和单纤维LDF可用于研究麻醉马匹后肢的血流。然而，需要进一步研究以确定股动脉血流变化为何与微血管灌注变化无关。
