Suzuki T, Ezure T, Yamaguchi T, Domen H, Ishida M, Schmidt W
Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.
J Pharm Pharmacol. 2000 Feb;52(2):243-51. doi: 10.1211/0022357001773760.
Procaine has been used to stimulate plant growth and it has been noted that it also promotes growth of microorganisms. The effect of procaine hydrochloride concentration on the growth rates of several species of microalgae and cyanobacteria was studied under both photoautotropic and heterotrophic growth conditions. Procaine hydrochloride was added to cultures at concentrations over the range 0.01-1000 mg L(-1). A stimulating effect of procaine hydrochloride on photoautotrophic growth was observed for the cyanobacteria Anabaena cylindrica and Anabaena variabilis, and for the salt-tolerant green algae Dunaliella primolecta and Dunaliella parva. During active growth in batch culture an increase in growth rate (compared with control culture without procaine hydrochloride) of about 25% was observed at 0.1 mgL(-1) of procaine hydrochloride for A. cylindrica. However, procaine hydrochloride was toxic at concentrations of > 10 mgL(-1). Simultaneous administration of hydrolysis products of procaine, p-aminobenzoic acid and diethyl aminoethanol, in lieu of procaine hydrochloride, was as effective as procaine in stimulating growth of A. cylindrica. Heterotrophic growth of Chlorella ellipsoidea and Prototheca zopfii was not stimulated by procaine hydrochloride over the concentration range studied (0.1-10 mg L(-1)). The combined effects of procaine hydrochloride concentration and four other environmental factors (temperature, light intensity, CO2 concentration in the flushing gas and NaCl concentration) on growth rate of D. primolecta was modelled using both a neural network approach and a response surface method. These results indicate that procaine hydrochloride exerts different effects on the growth of microalgal and cyanobacterial cells as functions of dosage, species and culture conditions.
普鲁卡因已被用于刺激植物生长，并且人们注意到它也能促进微生物的生长。在光自养和异养生长条件下，研究了盐酸普鲁卡因浓度对几种微藻和蓝藻生长速率的影响。将盐酸普鲁卡因以0.01 - 1000 mg L⁻¹的浓度添加到培养物中。观察到盐酸普鲁卡因对蓝藻圆柱鱼腥藻和多变鱼腥藻以及耐盐绿藻原始杜氏藻和小球杜氏藻的光自养生长有刺激作用。在分批培养的活跃生长阶段，对于圆柱鱼腥藻，在0.1 mgL⁻¹的盐酸普鲁卡因浓度下观察到生长速率（与不含盐酸普鲁卡因的对照培养物相比）增加了约25%。然而，盐酸普鲁卡因在浓度> 10 mgL⁻¹时具有毒性。同时施用普鲁卡因的水解产物对氨基苯甲酸和二乙氨基乙醇来代替盐酸普鲁卡因，在刺激圆柱鱼腥藻生长方面与普鲁卡因一样有效。在所研究的浓度范围（0.1 - 10 mg L⁻¹）内，盐酸普鲁卡因未刺激椭圆小球藻和佐夫氏原藻的异养生长。使用神经网络方法和响应面法对盐酸普鲁卡因浓度与其他四个环境因素（温度、光照强度、冲洗气体中的CO₂浓度和NaCl浓度）对原始杜氏藻生长速率的联合影响进行了建模。这些结果表明，盐酸普鲁卡因根据剂量、物种和培养条件对微藻和蓝藻细胞的生长产生不同的影响。
