Lõivukene K, Kolk H, Maaroos H I, Kasenõmm P, Ustav M, Mikelsaar M
Department of Microbiology, University of Tartu, Estonia.
Scand J Infect Dis. 2000;32(1):59-62. doi: 10.1080/00365540050164236.
The prevalence of metronidazole and clarithromycin resistance of Helicobacter pylori strains under different growth conditions (microaerophilic or anaerobic preincubation) was tested in 56 patients suffering from gastritis and peptic ulcer. vacA subtypes were detected in 46 H. pylori strains and were subsequently compared with the antibiotic resistance pattern. From 56 isolates, 26 proved resistant and 30 sensitive to metronidazole. The patients with peptic ulcer and gastritis were infected with both metronidazole-sensitive and metronidazole-resistant strains. In anaerobic preincubation all the strains were sensitive to metronidazole (MIC < 8 mg/l). All the strains were clarithromycin-sensitive (MIC < 2 mg/l). In the patients with gastritis and peptic ulcer s1 was the predominant vacA subtype. Comparison of vacA subtypes with the diagnoses revealed no correlation; different virulence factors such as vacA subtypes and antibiotic resistance to metronidazole in a microaerophilic milieu proved unrelated.
在56例胃炎和消化性溃疡患者中，检测了不同生长条件（微需氧或厌氧预培养）下幽门螺杆菌菌株对甲硝唑和克拉霉素的耐药率。在46株幽门螺杆菌菌株中检测到vacA亚型，随后将其与抗生素耐药模式进行比较。在56株分离株中，26株对甲硝唑耐药，30株敏感。消化性溃疡和胃炎患者感染的既有对甲硝唑敏感的菌株，也有耐药菌株。在厌氧预培养中，所有菌株对甲硝唑敏感（MIC<8mg/l）。所有菌株对克拉霉素敏感（MIC<2mg/l）。在胃炎和消化性溃疡患者中，s1是主要的vacA亚型。将vacA亚型与诊断结果进行比较，未发现相关性；在微需氧环境中，不同的毒力因子如vacA亚型和对甲硝唑的抗生素耐药性被证明是不相关的。
