Hosseini Razieh Sadat, Rahimian Ghorbanali, Shafigh Mohammad Hadi, Validi Majid, Khaledi Mansoor, Gholipour Abolfazl
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Internal Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
AMB Express. 2021 Nov 3;11(1):147. doi: 10.1186/s13568-021-01310-9.
The purpose of this study was to determine the mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from biopsy samples that were collected from the endoscopic ward of Shahrekord Hajar teaching Hospital and also to study the frequency of virulence factor and their correlation and pathological findings with clarithromycin resistance during the years 2019-2020. In this cross-sectional descriptive study, 152 patients with Helicobacter pylori infection were considered, and then, two common A2142G and A2143G mutations in the 23SrRNA gene associated with resistance were analyzed by Real-time PCR (Taq man). The presence of vacA, iceA1, iceA2, cagA, babA2, and oipA virulence genes was investigated by PCR and electrophoresis in 8% polyacrylamide gel. Then, data were analyzed using the relevant statistical tests. In this study, the frequency of Helicobacter pylori was 76% and the frequency of mutant isolates was 57.2%. The frequencies of A2142G and A2143G point mutations were 42.1% and 28.3%. There was a significant correlation among oipA, vacA, and iceA1 virulence factors, type of disease, chronic inflammatory score, and glandular atrophy with the antibiotic resistance to clarithromycin. There was no significant correlation between the age and sex of the patients with antibiotic resistance. According to the results of this study, it seems that the use of clarithromycin to combat this bacterium should be limited.
本研究的目的是确定从沙赫雷库尔德哈贾尔教学医院内镜病房采集的活检样本中分离出的幽门螺杆菌菌株与克拉霉素耐药性相关的突变，并研究2019 - 2020年期间毒力因子的频率及其与克拉霉素耐药性的相关性以及病理结果。在这项横断面描述性研究中，纳入了152例幽门螺杆菌感染患者，然后通过实时荧光定量PCR（Taq man法）分析与耐药性相关的23SrRNA基因中两个常见的A2142G和A2143G突变。通过PCR及8%聚丙烯酰胺凝胶电泳检测vacA、iceA1、iceA2、cagA、babA2和oipA毒力基因的存在情况。然后，使用相关统计检验对数据进行分析。在本研究中，幽门螺杆菌的感染率为76%，突变菌株的频率为57.2%。A2142G和A2143G点突变的频率分别为42.1%和28.3%。oipA、vacA和iceA1毒力因子、疾病类型、慢性炎症评分和腺体萎缩与对克拉霉素的抗生素耐药性之间存在显著相关性。患者的年龄和性别与抗生素耐药性之间无显著相关性。根据本研究结果，似乎应限制使用克拉霉素来对抗这种细菌。
