Tsuge H, Hotta N, Hayakawa T
Department of Food Science, Faculty of Agriculture, Gifu University, Yanagido, Japan.
J Nutr. 2000 Feb;130(2S Suppl):333S-334S. doi: 10.1093/jn/130.2.333S.
To investigate interactions between vitamin B-6 and fatty acid metabolism, male Wistar rats were fed a vitamin B-6 (B-6)-deficient diet consisting of 70% vitamin-free casein and 10% perilla oil [approximately 63% alpha-linolenic acid, (n-3)] for 5 wk. The amounts of linoleic acid (n-6) and arachidonic acid (n-6) in the B-6-deficient group changed only slightly compared with those in a pair-fed control group. The amount of linoleic acid increased and arachidonic acid decreased in the plasma total lipid fraction, and the ratios of both eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the B-6-deficient group were significantly lower than for the controls. The ratios of alpha-linolenic acid and EPA were higher, and DHA lower, in the B-6-deficient group than in the pair-fed control group in the total lipid as well as phospholipid fractions in liver microsomes. The activity of delta6-desaturase was significantly lower in the B-6-deficient group than in the pair-fed control group (approximately 64%), and acyl-CoA oxidase activity, an initial enzyme of the peroxisomal beta-oxidation pathway, was reduced by approximately 80% in the B-6-deficient group. These data suggest that B-6 deficiencies impair the metabolism of (n-3) PUFA from alpha-linolenic acid to EPA and DHA with the most pronounced reduction in the production of DHA.
为研究维生素B-6与脂肪酸代谢之间的相互作用，给雄性Wistar大鼠喂食缺乏维生素B-6（B-6）的日粮，该日粮由70%无维生素酪蛋白和10%紫苏油[约63%α-亚麻酸，（n-3）]组成，持续5周。与配对喂食对照组相比，B-6缺乏组中亚油酸（n-6）和花生四烯酸（n-6）的含量变化很小。在血浆总脂质部分，亚油酸含量增加，花生四烯酸含量减少，并且B-6缺乏组中二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的比例均显著低于对照组。在肝脏微粒体的总脂质以及磷脂部分中，B-6缺乏组中α-亚麻酸与EPA的比例较高，而DHA的比例低于配对喂食对照组。B-6缺乏组中δ6-去饱和酶的活性显著低于配对喂食对照组（约64%），并且过氧化物酶体β-氧化途径的起始酶酰基辅酶A氧化酶活性在B-6缺乏组中降低了约80%。这些数据表明，B-6缺乏会损害从α-亚麻酸到EPA和DHA的（n-3）多不饱和脂肪酸（PUFA）代谢，其中DHA的生成减少最为明显。
