Francino M P, Ochman H
Department of Biology, University of Rochester.
Mol Biol Evol. 2000 Mar;17(3):416-22. doi: 10.1093/oxfordjournals.molbev.a026321.
Certain mutations are known to occur with differing frequencies on the leading and lagging strands of DNA. The extent to which these mutational biases affect the sequences of higher eukaryotes has been difficult to ascertain because the positions of most replication origins are not known, making it impossible to distinguish between the leading and lagging strands. To resolve whether strand biases influence the evolution of primate sequences, we compared the substitution patterns in noncoding regions adjacent to an origin of replication identified within the beta-globin complex. Although there was limited asymmetry around the beta-globin origin of replication, patterns of substitutions do not support the existence of a mutational bias between the leading and lagging strands of chromosomal DNA replication in primates.
已知某些突变在DNA的前导链和后随链上出现的频率不同。由于大多数复制起点的位置未知，因此难以确定这些突变偏向在多大程度上影响高等真核生物的序列，这使得无法区分前导链和后随链。为了确定链偏向是否影响灵长类序列的进化，我们比较了β-珠蛋白复合体中确定的复制起点附近非编码区的替换模式。尽管β-珠蛋白复制起点周围的不对称性有限，但替换模式并不支持灵长类染色体DNA复制的前导链和后随链之间存在突变偏向。
