抗真菌咪唑类药物酮康唑和咪康唑对培养的白纹伊蚊（C6/36）蚊细胞中登革病毒复制的刺激作用。

Stimulation of dengue virus replication in cultured Aedes albopictus (C6/36) mosquito cells by the antifungal imidazoles ketoconazole and miconazole.

作者信息

Lee E, McLean K, Weir R C, Dalgarno L

机构信息

Division of Biochemistry and Molecular Biology, Australian National University, Canberra, ACT, 0200, Australia.

出版信息

Virology. 2000 Mar 30;269(1):1-6. doi: 10.1006/viro.2000.0217.

DOI:10.1006/viro.2000.0217

PMID:10725192

Abstract

Replication of dengue type 3 virus in Aedes albopictus C6/36 cells was enhanced more than 50-fold by addition of the antifungal imidazole derivative ketoconazole within the first 4 h of infection. The stimulatory effect was reflected in the yield of infectious virus and in levels of viral RNA and protein synthesis. Enhanced yields were observed also for other flaviviruses, including dengue type 2 virus and Murray Valley encephalitis virus. Increased yields of dengue type 3 virus were not observed in African green monkey kidney (Vero) cells, human monocytic (U-937) cells, or cells of the mosquito Toxorhynchites amboinensis (TRA-171).

摘要

在白纹伊蚊C6/36细胞中，在感染的最初4小时内添加抗真菌咪唑衍生物酮康唑，登革3型病毒的复制增强了50多倍。这种刺激作用体现在感染性病毒的产量以及病毒RNA和蛋白质合成水平上。对于其他黄病毒，包括登革2型病毒和墨累谷脑炎病毒，也观察到产量增加。在非洲绿猴肾（Vero）细胞、人单核细胞（U-937）细胞或蚊种amboinensis（TRA-171）的细胞中未观察到登革3型病毒产量增加。