大规模cDNA分析揭示了小鼠植入前发育过程中的阶段性基因表达模式。

Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse development.

作者信息

Ko M S, Kitchen J R, Wang X, Threat T A, Wang X, Hasegawa A, Sun T, Grahovac M J, Kargul G J, Lim M K, Cui Y, Sano Y, Tanaka T, Liang Y, Mason S, Paonessa P D, Sauls A D, DePalma G E, Sharara R, Rowe L B, Eppig J, Morrell C, Doi H

机构信息

ERATO Doi Bioasymmetry Project, JST, Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI 48202, USA.

出版信息

Development. 2000 Apr;127(8):1737-49. doi: 10.1242/dev.127.8.1737.

DOI:10.1242/dev.127.8.1737

PMID:10725249

Abstract

Little is known about gene action in the preimplantation events that initiate mammalian development. Based on cDNA collections made from each stage from egg to blastocyst, 25438 3'-ESTs were derived, and represent 9718 genes, half of them novel. Thus, a considerable fraction of mammalian genes is dedicated to embryonic expression. This study reveals profound changes in gene expression that include the transient induction of transcripts at each stage. These results raise the possibility that development is driven by the action of a series of stage-specific expressed genes. The new genes, 798 of them placed on the mouse genetic map, provide entry points for analyses of human and mouse developmental disorders.

摘要

关于启动哺乳动物发育的植入前事件中的基因作用，我们所知甚少。基于从卵子到囊胚各个阶段所制备的cDNA文库，获得了25438个3'-ESTs，代表9718个基因，其中一半是新基因。因此，相当一部分哺乳动物基因致力于胚胎表达。这项研究揭示了基因表达的深刻变化，包括每个阶段转录本的瞬时诱导。这些结果增加了发育是由一系列阶段特异性表达基因的作用所驱动的可能性。这些新基因中有798个已定位到小鼠遗传图谱上，为分析人类和小鼠发育障碍提供了切入点。