前沿：孤儿趋化因子受体G蛋白偶联受体-2（GPR-2，CCR10）与皮肤相关趋化因子CCL27（CTACK/ALP/ILC）结合。

Cutting edge: the orphan chemokine receptor G protein-coupled receptor-2 (GPR-2, CCR10) binds the skin-associated chemokine CCL27 (CTACK/ALP/ILC).

作者信息

Homey B, Wang W, Soto H, Buchanan M E, Wiesenborn A, Catron D, Müller A, McClanahan T K, Dieu-Nosjean M C, Orozco R, Ruzicka T, Lehmann P, Oldham E, Zlotnik A

机构信息

DNAX Research Institute, Palo Alto, CA 94304, USA.

出版信息

J Immunol. 2000 Apr 1;164(7):3465-70. doi: 10.4049/jimmunol.164.7.3465.

DOI:10.4049/jimmunol.164.7.3465

PMID:10725697

Abstract

We recently reported the identification of a chemokine (CTACK), which has been renamed CCL27 according to a new systematic chemokine nomenclature. We report that CCL27 binds the previously orphan chemokine receptor GPR-2, as detected by calcium flux and chemotactic responses of GPR-2 transfectants. We renamed this receptor CCR10. Because of the skin-associated expression pattern of CCL27, we focused on the expression of CCL27 and CCR10 in normal skin compared with inflammatory and autoimmune skin diseases. CCL27 is constitutively produced by keratinocytes but can also be induced upon stimulation with TNF-alpha and IL-1beta. CCR10 is not expressed by keratinocytes and is instead expressed by melanocytes, dermal fibroblasts, and dermal microvascular endothelial cells. CCR10 was also detected in T cells as well as in skin-derived Langerhans cells. Taken together, these observations suggest a role for this novel ligand/receptor pair in both skin homeostasis as well as a potential role in inflammatory responses.

摘要

我们最近报道了一种趋化因子（CTACK）的鉴定，根据新的趋化因子系统命名法，它已被重新命名为CCL27。我们报道，通过GPR-2转染细胞的钙流和趋化反应检测发现，CCL27与之前未明确配体的趋化因子受体GPR-2结合。我们将该受体重新命名为CCR10。由于CCL27的表达模式与皮肤相关，我们重点研究了与炎症性和自身免疫性皮肤病相比，CCL27和CCR10在正常皮肤中的表达情况。角质形成细胞可组成性产生CCL27，但在肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）刺激后也可被诱导产生。角质形成细胞不表达CCR10，相反，黑素细胞、真皮成纤维细胞和真皮微血管内皮细胞表达CCR10。在T细胞以及皮肤来源的朗格汉斯细胞中也检测到了CCR10。综上所述，这些观察结果表明，这种新型配体/受体对在皮肤稳态以及炎症反应中可能发挥作用。