Blakytny R, Jude E B, Martin Gibson J, Boulton A J, Ferguson M W
School of Biological Sciences, University of Manchester, Stopford Building, Manchester, UK.
J Pathol. 2000 Apr;190(5):589-94. doi: 10.1002/(SICI)1096-9896(200004)190:5<589::AID-PATH553>3.0.CO;2-T.
Wound healing, including re-epithelialization, is delayed in diabetes. Growth factors influence the healing process and amongst these, insulin-like growth factor (IGF) has been shown to stimulate keratinocyte proliferation in vitro. Monoclonal antibodies to insulin-like growth factors 1 and 2 (IGF1 and IGF2) were used to investigate their distribution in diabetic foot ulcers and surrounding tissues by immunohistochemistry, compared with diabetic and non-diabetic uninjured skin. IGF2 was found throughout the epidermis (stratum granulosum, spinosum, and basale) in all three groups. Staining for IGF2 was intense in both normal and diabetic skin as well as in diabetic foot ulcers, being greatest at the ulcer edge. IGF1, in comparison, was found throughout the epidermis of non-diabetic skin; expression was restricted to the stratum granulosum and spinosum of uninjured diabetic skin and was absent in the basal layer at the ulcer edge. A similar absence of IGF1 in dermal fibroblasts was found in tissue sections from diabetic patients. This lack of expression of IGF1 within the basal layer and fibroblasts may contribute to retarded wound healing in diabetes mellitus.
伤口愈合，包括再上皮化，在糖尿病患者中会延迟。生长因子会影响愈合过程，其中胰岛素样生长因子（IGF）已被证明在体外能刺激角质形成细胞增殖。通过免疫组织化学方法，使用胰岛素样生长因子1和2（IGF1和IGF2）的单克隆抗体来研究它们在糖尿病足溃疡及其周围组织中的分布，并与糖尿病和非糖尿病未受伤皮肤进行比较。在所有三组中，IGF2均存在于整个表皮（颗粒层、棘层和基底层）。IGF2在正常皮肤和糖尿病皮肤以及糖尿病足溃疡中均有强烈染色，在溃疡边缘处最为明显。相比之下，IGF1存在于非糖尿病皮肤的整个表皮中；在未受伤的糖尿病皮肤中，其表达仅限于颗粒层和棘层，在溃疡边缘的基底层中不存在。在糖尿病患者的组织切片中，真皮成纤维细胞中也发现类似的IGF1缺失情况。基底层和成纤维细胞中IGF1的这种表达缺失可能导致糖尿病患者伤口愈合延迟。
