Pappa A, Seferiadis K, Marselos M, Tsolas O, Messinis I E
Department of Pharmacology, University of Ioannina Medical School, Greece.
Theriogenology. 1999 Apr 1;51(5):911-26. doi: 10.1016/s0093-691x(99)00038-2.
Rat LH (rLH) and FSH (rFSH) were measured by sensitive and specific competition ELISAs. The rat LH ELISA used rLH-I-9 coated plates, an antiserum against rLH and an antibody against rabbit IgG labeled with peroxidase. Using rLH-RP-3 as a standard, rat LH was determined by binding of the anti-LH antibody to rLH-I-9 coated plates. The sensitivity of the assay was 0.8 ng/mL. Similarly, the rat FSH-ELISA used rFSH-I-8 coated plates, an antiserum against rFSH and an antibody against rabbit IgG labeled with peroxidase. Using rFSH-RP-3 as a standard, the FSH-ELISA was also determined by binding of the anti-FSH antibody to rFSH-I-8 coated plates. The sensitivity of this assay was 1.25 ng/mL. Both rat LH and FSH ELISA assays are highly specific and provide accurate determination of gonadotrophins in buffers, sera, cell culture media, and anterior pituitary extracts. These assays were used for monitoring the gonadotrophin surge-attenuating factor (GnSAF) and inhibin activities present in human follicular fluid (hFF). The 2 new ELISA procedures have practical advantages (safety, convenience, economy) over the RIA methods, and they perform as well as the RIA techniques at the same range of concentrations.
采用灵敏且特异的竞争酶联免疫吸附测定法（ELISA）检测大鼠促黄体生成素（rLH）和促卵泡生成素（rFSH）。大鼠LH ELISA使用包被有rLH-I-9的酶标板、抗rLH抗血清以及用辣根过氧化物酶标记的抗兔IgG抗体。以rLH-RP-3作为标准品，通过抗LH抗体与包被有rLH-I-9的酶标板结合来测定大鼠LH。该检测方法的灵敏度为0.8 ng/mL。同样，大鼠FSH-ELISA使用包被有rFSH-I-8的酶标板、抗rFSH抗血清以及用辣根过氧化物酶标记的抗兔IgG抗体。以rFSH-RP-3作为标准品，FSH-ELISA也是通过抗FSH抗体与包被有rFSH-I-8的酶标板结合来测定。该检测方法的灵敏度为1.25 ng/mL。大鼠LH和FSH ELISA检测方法均具有高度特异性，能够准确测定缓冲液、血清、细胞培养基和垂体前叶提取物中的促性腺激素。这些检测方法用于监测人卵泡液（hFF）中存在的促性腺激素激增衰减因子（GnSAF）和抑制素活性。这两种新的ELISA方法相对于放射免疫分析（RIA）方法具有实际优势（安全性、便利性、经济性），并且在相同浓度范围内与RIA技术表现相当。
